Blastodisc cap formation occurred in all embryos treated with 0.
These results suggest that a microtubule-associated mechanism is responsible for ooplasm segregation and blastodisc formation in the squid.
All of the eggs laid by the five virgin females, the four second-clutch females, and the three females allowed to mate with vasectomised males, were "collapsed" at oviposition, and none showed any sign of a blastodisc or its associated vascular rosette.
Thus, we conclude that unfertilised eggs of sand lizards are easily recognizable at laying: they lack turgot and lack blastodiscs and the associated vascularity.
marulius eggs, blastodisc
divided after 35-60 min into two blastomeres.
Exposure to cold inhibited blastodisc cap formation in all embryos treated prior to cytoplasmic streaming; it also arrested streaming in embryos treated after second polar body formation.
These results suggest that the ordered movement of cytoplasm, which forms the blastodisc in the squid, is disturbed by cold treatment.
In these eggs, the blastodisc has begun to divide into two blastomeres, and essentially all oil droplets have aggregated in a crude ring near the vegetal pole.
2+] gradients near the poles, and to inhibit the formation of the blastodisc and the movement of oil droplets toward the vegetal pole (Fluck et al.
The volume of the blastodisc
was measured with an image analysis program (Microcomp Planar Morphometry, Southern Micro Instruments; Abraham et al.