blotting

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blotting

[′bläd·iŋ]
(cell molecular)
The transfer of electrophoretically separated polypeptides onto a solid support medium, such as nitrocellulose paper or a nylon membrane.
References in periodicals archive ?
c) Western blot analysis of KLF-4 in LA-4 cells after the treatment with 0, 1, 2, 5, or 10 ng/mL TGF-[beta] for 24 hours; each KLF-4 value was expressed as percentage to [beta]-actin.
Western blot analysis of AT1R and AT2R was performed (Figure 8(a)).
To examine the protein levels of antioxidants in the liver, the animals ( n = 7 in each group) were used for western blot analysis according to the method of our previous study.
Downregulation of PCNA and calbindin-D9k protein levels after BPA exposure were confirmed by Western blot analysis (see Supplemental Material, Figure S1B,C).
Sc] in skeletal and heart muscle by Western blot analysis with sodium phosphotungstic acid precipitation for enrichment of [PrP.
Western blot analysis of tissue homogenates showed that MDM2 protein levels were decreased by 25-OC[H.
One key hurdle to the implementation of a population-based carrier screen for FXS is technical: The conventional approach for detecting FMR1 expansions involves a PCR-based assay, which is supplemented by Southern blot analysis because of the high GC content of the FMR1 repeats and the large sizes of disease-causing expansions.
Methodology: Immunohistochemistry and Western blot analysis were applied to detect expression of EGFR and EGFR vIII in specimens of esophageal carcinoma patients.
Western blot analysis of IRF-3 was performed on cell lines expressing several types of HPV.