The system needs to be compatible with non-transparent chips for both bright-field
images and fluorescent images of attached tissue sections.
The Opterra II also features a sub-10% field illumination uniformity; options for bypass capability for wide-field, bright-field
, and TIRF imaging; a high-speed 10-position filter wheel; as well as photo-activation, spectral imaging and un-mixing capabilities.
HER2 dual in situ hybridization (DISH) assay vs fluorescence in situ hybridization (FISH): focused study of immunohistochemical 2+ cases.
The original guideline focused on IHC and fluorescence in-situ hybridization (FISH), whereas the updated guideline adds recommendations for a newer diagnostic technique known as bright-field
These enhancements include the combining fluorescent and bright-field
pathology analysis with both IHC and FISH imaging and analysis in a single platform.
In this activity, students will (1) learn the applications and use of ImageJ, (2) learn to measure cell area from a bright-field
image and compute data to reach conclusions, and (3) explore on their own the use of the cell-counter plug-in for ImageJ.
Particle-size distributions were obtained by manual measurement of particle diameters from bright-field
In a simpler and more straightforward alternative, the problem of autofluorescence can be avoided altogether by basing detection on enzymatic development of a substrate and visualizing the locations of the reaction products with bright-field
5% neutral red solution (Carlo Erba, Milan, Italy) for 25 min, dehydrated in ethanol (50-100%) and xylene, and mounted with DPX (p-xylene-bis[N-pyridinium bromide]; Sigma) for observations with a bright-field
Dialux EB 20 microscope (Leitz, Stuttgart, Germany).
Their topics include immunostaining techniques for the co-localization of multiple peptide anginas in light microscopy, gold cluster labels and related technologies, high throughput morphological gene expression studies using automated mRNA in situ hybridization applications and tissue microarrays for post-genomic and clinical research, and the three-dimensional full color demonstration of bright-field
and fluorescence microscopic preparations using the digital optical microscope.
For conventional bright-field
morphology observation, the obtained TEM specimen was shadowed with Pt/C in a high-vacuum evaporator to achieve high-contrast images.
Semithin (1 to 2 pm) sections were cut with glass knives on an ultramicrotome and stained with toluidine blue and basic fuchsin for light microscopic examination with bright-field
and differential interference contrast optics.