bright-field

bright-field

[′brīt ‚fēld]
(optics)
Having a brightly lighted background.
References in periodicals archive ?
The researchers developed a novel algorithm that can recover the phase information from a stack of bright-field images taken by a classical microscope.
RNAscope is compatible with routine formalin-fixed, paraffin-embedded tissue specimens and can use either conventional chromogenic dyes for bright-field microscopy or fluorescent dyes for multiplex analysis.
The eyepieces are suited for use with standard achromatic objectives and upright, bright-field microscope systems.
As such, our group has developed and previously published data on a second-generation automated bright-field dualcolor in situ hybridization assay for MDM2.
Z1 is a whole slide scanner for bright-field and fluorescence samples that is utilized to record virtual slides of tissue sections in research and clinical pathology labs around the world.
This system consists of two technologies, such as bright-field inspection and dark-field inspection.
The Opterra II also features a sub-10% field illumination uniformity; options for bypass capability for wide-field, bright-field, and TIRF imaging; a high-speed 10-position filter wheel; as well as photo-activation, spectral imaging and un-mixing capabilities.
Thus, one method to obtain a 3-D color image is to do a two-scan measurement: one scan with an interferometric objective delivering 3-D measurement data based on finding a best focus position for each pixel, and a second scan with a bright-field objective during which imaging information is collected only at the previously predetermined best-focus positions and then overlaid on the 3-D measurement data.
The technology is compatible with FFPE specimens, conventional chromogenic and fluorescent dyes, and is suitable for bright-field microscopy and multiplex analysis.
In this activity, students will (1) learn the applications and use of ImageJ, (2) learn to measure cell area from a bright-field image and compute data to reach conclusions, and (3) explore on their own the use of the cell-counter plug-in for ImageJ.
Particle-size distributions were obtained by manual measurement of particle diameters from bright-field TEM micrographs.
In a simpler and more straightforward alternative, the problem of autofluorescence can be avoided altogether by basing detection on enzymatic development of a substrate and visualizing the locations of the reaction products with bright-field microscopy.