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chaotropic |
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chaotropic [‚kā·ə′träp·ik] (bichemistry) Having the ability to destabilize hydrogen bonding and hydrophobic interactions. How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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DNA was extracted from serum (n=4), blood (n=25), blood culture (n=9), and bone marrow (n=2) specimens by using the chaotropic properties of guanidine isothiocyanate, followed by DNA extraction and purification by alcohol precipitation with the IsoQuick Nucleic Acid Extraction Kit (ORCA Research, Bothell, WA). ChargeSwitch([R]) Technology was specifically developed to address the limitations of current purification technologies and is the only sample preparation method that does not use ethanol or chaotropic salts, two reagents that are known to inhibit enzymatic reactions and present storage, shipping, and handling challenges. 2 g of the tissue pellet was digested by mechanical disruption and the DNA was purified by adsorption to glass milk in the presence of a chaotropic agent, according to the manufacturer's instructions (Fast Prep, Bio 101, Vista, CA). |
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