chaotropic


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chaotropic

[‚kā·ə′träp·ik]
(bichemistry)
Having the ability to destabilize hydrogen bonding and hydrophobic interactions.
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References in periodicals archive ?
23-25) In this case, by utilizing chaotropic conditions generated within the collected sample, human cells are selectively lysed, after which, enzyme-mediated digestion of human DNA and extracellular pathogen DNA occurs, followed by sedimentation of intact pathogen cells with the aid of centrifugation.
New technologies for diagnosing bloodstream infection and measuring antimicrobial resistance
Rna Extraction Is Based On Lysis Using A Chaotropic Buffer Containing Guanidine Thiocynate And Using Silica As The Rna-Binding Matrix.
Supply Of An Automated Magnetic Rna Extraction System
Briefly, specimens were digested under denaturing conditions at elevated temperatures and then lysed in the presence of chaotropic reagent.
A Comparison of the Roche Cobas HPV Test With the Hybrid Capture 2 Test for the Detection of High-Risk Human Papillomavirus Genotypes
In the following step, the lysis fluid containing DNA/RNA is transferred to a chaotropic buffer containing fresh silica beads.
Commercially available kits for manual and automatic extraction of nucleic acids from formalin-fixed, paraffin-embedded (FFPE) tissues
In brief, the sputum samples were mixed with 2-3 volumes of the USP solution, which contained the following chemicals: 4-6 M guanidinum hydrochloride (a chaotropic agent which disrupts the hydrogen bonds), 50 mM Tris chloride (pH 7.
Comparision between different conventional methods for the diagnosis of mycobacterium tuberculosis
Given our observed inability to greatly alter soy flour adhesion using chaotropic agents, salts, surfactants, or co-solvents (Frihart and Lorenz 2013), which is in contrast to the literature observations with soy protein isolate (Sun 2005b), we concluded that the carbohydrates were suppressing protein alteration for improved adhesion.
Soy flour adhesive strength compared with that of purified soy proteins
Chaotropic acids such as guanidine hydrochloride or guanidinium thiocyanate were discovered to protect NA from nucleases because of their potent protein denaturing properties (16).
DNA/RNA preparation for molecular detection
2012)5 briefly 200l of each sample (nasopharyngeal aspirates or throat swabs) was added to 400l lysis/binding buffer containing a chaotropic salt poly(A) and proteinase K and then incubated for 45 minutes.
Identification of genotypes of Influenza A virus in Malaysia
TEST PARAMETERS Inclusion Body Wash Solubilization Refold Buffer composition Buffer composition Buffer composition * Chaotropic agents * Chaotropic agents * Chaotropic agents * Detergents * Detergents * Redox agents * Salts * Reducing agents * Amino Acids * Detergents * Sugars * Salts w/v ratio Duration Duration Cycles of Protein Protein centrifugation concentration concentration pH w/v raio
Streamlining recombinant protein production: the Corynex platform offers an alternative to traditional synthesis and purification processes
Chaotropic anions favour the formation of complex more than kosmotropic anions this is because chaotropes have low surface tension values as compared to kosmotropes which lead to strengthening of hydrophobic interaction and enhances the complex formation which in turn shift the LCST toward lower temperature.
The Influence of Different Ions on the Phase Separation of Poly (Vinyl Alcohol) and Poly (Sodium 4-Styrene Sulphonate) in Aqueous Solution
Kosmotropic (Glycerol) and chaotropic (NaCl) salt ions are mostly used to create water stress which either disrupt or enhance hydrogen bonding networks (Collins & Washabaugh, 1985; Cacace, Landau & Ramsden, 1997).
Isolation and characterization of osmotolerant bacteria from Thar Desert of Western Rajasthan (India)
The native state of a protein can be altered using elevated temperature, chaotropic or other chemical agents such as urea or guanidine hydrochloride, and adjustments in pH.
Modular spectroscopy tools for measuring intrinsic protein fluorescence: the availability of powerful LED UV light sources, when combined with spectroscopy methods, is opening up a new world of fluorescence measurements

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