A preliminary comparison of restriction fragment patterns in the genus Caulerpa (Chlorophyta) and the unique structure of the chloroplast genome
of Caulerpa sertularioides.
Yarborough Foundation funding will also be used for another of Mangum's projects--mapping the fast plant chloroplast genome
Specific topics encompass the paleobotany of Livingston Island, Antarctica; targeted isolation and sequence assembly and characterization of the white spruce; the complete chloroplast genome
sequence of a tree fern; reverse genetic analyses of gene function in fern gametophytes; glossopterid seed ferns from the Late Permian period; pollen development; DNA barcoding in the Cycadales; expressed sequence tag analysis in Ginkgo biloba; frequent fires in ancient shrub tundra; the history of native plant communities in the South; past vegetation patterns of New Mexico's Rio Del Oso Valley; and East Asian monsoon and paleoclimatic data analysis.
Yet the chloroplast genome
doesn't contain all the necessary genes to make the light-harvesting factories run; genes from the algae's nucleus are also required.
This began with the pioneering work of Jeffrey Palmer and Diana Stein who demonstrated the utility of variation in the chloroplast genome
for evolutionary studies in land plants, including ferns (Palmer, 1987; Palmer and Stein, 1982; Stein et al.
Genes derived from the mitochondrial genome should branch most closely with genes from alpha-Proteo-bacteria, and genes derived from the chloroplast genome
should branch most closely with cyanobacterial genes.
The psbA gene, located on chloroplast genome
and coding for Dl protein.
Conservation of chloroplast genome
structure among vascular plants.
One of the markers used was a thirty-two base pair repeat in the chloroplast genome
, while the other was a band produced by an AFLP analysis of the genomic DNA from different strawberry plant populations.
4 kb inversion in the chloroplast genome
in Joinvillea and the grasses; reduction to 1 ovule per pistil, gain of a lateral "grass-type" embryo, and gain of an inversion around the gene trnT in the chloroplast genome
in the grasses; loss of arm cells in the clade that consists of Brachyelytrum, Pooideae, and the PACC clade; loss of the epiblast and gain of an elongate mesocotyl internode in the PACC clade; gain of proximal female-sterile florets in female-fertile spikelets, gain of overlapping embryonic leaf margins, and gain of Panicum-type endosperm starch grains in the clade that comprises Centothecoideae and Panicoideae; and loss of the scutellar tail of the embryo in Pooideae (in one of two alternative placements of Pooideae among other groups).
The rbcL gene is a single copy chloroplast-encoded gene which, like the 18S rDNA, gives an intense signal due to the multicopy nature of the chloroplast genome
The gene for the leusine transfer RNA (trnL) from the chloroplast genome
(cpDNA) was amplified using polymerase chain reaction (PCR).