9 [micro]M) were the most potent compounds in this group; aclarubicin hydrochloride, plicamycin, and the structurally related chromomycin
A3 were also active, whereas adriamycin hydrochloride was inactive.
The GC and AT rich regions were detected using the fluorochromes Chromomycin
Several tests such as TdT (terminal deoxyribonucleotidyl transferase)-mediated dUTP nick-end labeling (TUNEL) assay, aniline blue straining, chromomycin A3 and acridine orange (AO) staining have been described to determine the extent of DNA damage.
Evaluation of sperm's chromatin quality with acridine orange test, chromomycin A3 and aniline blue staining in couples with unexplained recurrent abortion.
Additionally, for detection of the AT- and GC- rich chromatin regions, some chromosomal preparations were stained with the fluorochromes 4'-6-diamidino-2-phenylindole (DAPI) and chromomycin
Staining with aniline blue, toludine blue, and chromomycin
A3 is used to identify sperm chromatin packaging defects.
The antiproliferative activity of these compounds was found to be comparable to that of known antitumor agents, including cisplatin, chromomycin
, cytosine arabinoside and 5-fluorouracil.
Credibility of chromomycin
A3 staining in prediction of fertility.
1 mL) were frozen and stored at -70[degress]C for later analysis of chromatin integrity by the sperm chromatin structure assay (SCSA; Evenson and Jost 2000) and for chromatin maturity by chromomycin
A3 (CMA) staining (Sakkas et al.
Also, we can use chromomycin
A3 (CMA3), toluidine blue (TB) or aniline blue (AB) to detect the sperm chromatin integrity/maturity (30, 31).
2000 Sperm count surrogate Anderson and Sharpe 2000 Abbreviations: CMA3 staining, chromomycin
A3 staining; COMET, single-cell gel electrophoresis assay; FISH, fluorescence in situ hybridization; SCSA, sperm chromatin structure assay; TUNEL, terminal deoxynucleotidyl trans-ferase-mediated dUTP-biotin end-labeling.
Other antibiotics such as chromomycins
(Dactinomycin and Plicamycin) and a miscellaneous group Mitomycin and Bleomycin are also used.