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05 cM) of the molecular map around the gene will be attained with the screening of a 2000 individual segregating population with AFLP markers Besides, a BAC genomic library and a cosmid library will be creened in order to identify clones positive for the markers linked to Ry to start the chromosome walking in the region and to find a BAC clone which contain molecular markers placed at both sides of the gene
Identification of the cystic fibrosis gene: chromosome walking and jumping.
2003) found that 930 gametes were sufficient to do chromosome walking and assemble a contiguous BAC sequence spanning the Q gene on the long arm of chromosome 5A.
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