restriction site

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restriction site

[ri′strik·shən ‚sīt]
(cell and molecular biology)
A sequence in a deoxyribonucleic acid molecule that can be cleaved with a specific restriction endonuclease.
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References in periodicals archive ?
Analysis of the S1/S2 cleavage site of FIPV sequences shows that it has much more variability, both within the narrow furin cleavage recognition motif (P4-P1) and in residues extending out of it (P8-P5 and P2'-P4') (Figure 2A).
Although the precise cleavage site was not established and the processing capacity was very inefficient, this singular report does raise the possibility that other endoproteases may be involved in the posttranslational maturation of proBNP.
We analyzed PSA heterogeneity by 2DE, MS, and amino acid sequencing and created a catalog of variants of purified PSA, including novel peptide forms of PSA with unique cleavage sites.
The cleavage site specificity of human prostate specific antigen for insulin-like growth factor binding protein-3.
The 385-bp amplification product contained both polymorphic (position 41) and constitutive (position 210) cleavage sites for MboII.
It seems that these antibodies recognized only PSA forms where the Lys145-Lys146 cleavage site is intact, such as in LNCaP PSA, which was used as an immunogen.
On the contrary, the cleavage site of the liquid vaccines contained genomic sequences characteristic of velogenic strains.
A DNA/RNA chimeric reporter substrate containing fluorescent and quencher dye molecules on opposite sides of the cleavage site is added to the reaction mixture and is cleaved as the DNAzyme forms during PCR amplification.
We also found 1 putative change in the C/anchC cleavage site of the polyprotein (Table 2); this mutation was observed also in the highly divergent African USUV strain (ArB1803; GenBank accession no.
In vivo assay and sequencing of the hemagglutinin protein cleavage site classified the virus as a North American genetic lineage of low pathogenicity for chickens.
To confirm that CASP1 cleavage of the steroid receptor was pivotal to ALL steroid resistance, researchers engineered a receptor that lacked the CASP1 cleavage site.