column chromatography


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Related to column chromatography: Thin layer chromatography

column chromatography

[′käl·əm ‚krō·mə′täg·rə·fē]
(analytical chemistry)
Chromatographic technique of two general types: packed columns usually contain either a granular adsorbent or a granular support material coated with a thin layer of high-boiling solvent (partitioning liquid); open-tubular columns contain a thin film of partitioning liquid on the column walls and have an opening so that gas can pass through the center of the column.
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reflexa contains several constituents that can be isolated by column chromatography.
Usual work-up followed by purification of the resulting crude material by column chromatography over silica gel afforded pure 2 (yield: 78%).
3] solution (10%) in methanol [9] to afford a 1:1 mixture of the diastereomeric keto-diols 9a/9b in 90% yield, which were separated by column chromatography through silica gel using a 1:1 mixture of n-hexane and ethyl acetate as eluent.
The remainder was purified on silica gel column chromatography with 1:5 ethyl acetate in hexanes as a mobile phase.
6% ethanol to have resveratrol/viniferin concentration around 10 mg/mL, and the solution was subjected to column chromatography on a polyamide carrier (Polyamide Woelm).
Some methods used for cleanup for the measurement of semivolatile analytes include column chromatography, thin-layer chromatography, sweep codistillation, and gel permeation chromatography.
The crude product was purified by Sephadex LH-20 column chromatography (hexane -- chloroform, 1:1 by volume) and stored at 4[degrees]C.
Many methods have been employed to quantify surfactants, including methylene blue active substance (MBAS), cobalt thiocyanate active substance (CTAS), high performance liquid chromatograph (HPLC) and column chromatography.
7 Retention Parameters in Column Chromatography of the Nomenclature for Chromatography in "Nomenclature for Chromatography (IUPAC Recommendations 1993)", with the aims of (i) emphasizing the physical meaning of the terms and (ii) specifying the temperatures and pressures for the terms for gas volumes and flow rates.