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column chromatography |
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column chromatography [′käl·əm ‚krō·mə′täg·rə·fē] (analytical chemistry) Chromatographic technique of two general types: packed columns usually contain either a granular adsorbent or a granular support material coated with a thin layer of high-boiling solvent (partitioning liquid); open-tubular columns contain a thin film of partitioning liquid on the column walls and have an opening so that gas can pass through the center of the column. How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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To analyze these proteins, they must be physically separated into discrete pools using different separation technologies (two-dimensional gel electrophoresis, column chromatography and others). Traditional column chromatography systems used to purify biopharmaceuticals require significant downtime and labor to disassemble components for routine maintenance, resulting in lost throughput. Sellick demonstrated the higher binding capacity of single-use Mustang(TM) membrane capsules for large molecule applications compared to conventional resin- or gel-based column chromatography. |
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