Fusing two single stranded DNA molecules by complementary base pairing
is also called annealing.
The students took on character roles of DNA and RNA while acting out complementary base pairing, splicing of introns and joining of exons, matching of mRNA codons to the corresponding amino acids, and assembly of amino acids on a growing polypeptide chain.
The students already knew about complementary base pairing from a previous chapter, so I had them collaborate in pairs to work on the base pairing for DNA, mRNA, and tRNA, noting the replacement of thymine (T) by uracil (U) in RNA.
However, Taq was eventually found to be susceptible to errors in complementary base pairing during primer extension (see section on PCR protocol); and although the Taq polymerase is still used, many investigators currently prefer to use the DNA polymeruse cloned from Pyrococcus furiosus (Pfu), an organism that grows optimally in geothermal marine sediments at 100 [degrees] C.
Complementary base pairing can only occur between the sense ssDNA strand and the antisense primer, and the antisense ssDNA strand and the sense primer [ILLUSTRATION FOR FIGURE 1 OMITTED].
Also known as complementary base pairing, the hydrogen bonding of both A to T and C to G is in a 1:1 ratio.
When linked by complementary base pairing, the two strands that comprise dsDNA are called sense and antisense strands, where the DNA antisense strand is the complementary base pair copy of the DNA sense strand [ILLUSTRATION FOR FIGURE 2 OMITTED].