confocal laser-scanning fluorescence microscopy

confocal laser-scanning fluorescence microscopy

[‚kan¦fō·kəl ‚lā·zər ‚skan·iŋ flə¦res·ənt mī′kräs·kə·pē]
(biophysics)
A technique that allows three-dimensional microscopic image sectioning of a specimen such as a cell by scanning the object step by step with a laser spot, instead of illuminating it as a whole. From each point the fluorescence is measured and, after analog-to-digital conversion, stored as a matrix in computer memory.