Positional cloning using interval-specific congenic strains narrowed this QTL to a 0.
Because the DBA/2J and C57BL/6J mice that were used to generate the interval-specific congenic strains differ significantly in the brain levels of some oxidative stress markers (Rebrin et al.
Classical congenic strains typically are derived from two inbred progenitor strains (i.
Interval-specific congenic strains (ISCS): An experimental design for mapping a QTL into a 1-centimorgan interval Mammalian Genome 8:163-167, 1997.
After repeated backcrosses, the congenic strains
carry the DNA of the recipient strain except for one relatively small piece of DNA that is still derived from the second parental inbred strain (i.
models called interval-specific congenic strains
[ISCS] and advanced intercross lines [AIL]) as well as of heterogeneous populations (see Palmer and Phillips 2002).
Using this strategy, one can produce several congenic strains
that carry different or overlapping parts of the original QTL.