CAT, as a catalase, is a kind of enzymes scavenger and conjugase
with prosthetic group of iron porphyrin.
Food folate assay with protease, a-amylase, and folate conjugase
To release bound folate, alpha-amylase, protease and conjugase
were added to the extracts.
While almost all of the monoglutamate is available for absorption, only 30% to 80% of the polyglutamates are available due in part to the presence of conjugase
inhibitors in certain plant foods (e.
Samples were removed every 3 hr, heat-treated and treated with conjugase
before the microbiological assay was performed.
It is assumed that this procedure achieves two goals: the complete lysis of erythrocytes and the exposure of intracellular folate polyglutamates to plasma conjugase
enzyme ([gamma]-glutamyl hydrolase) at a pH near 4.
All lysates (including those that may have undergone preliminary freezing-thawing) were incubated for 2 h at 37 [degrees]C so that any released red cell folyl-polyglutamates could undergo deconjugation, with naturally occurring plasma folate conjugase
(y-Glu-X carboxypeptidase; EC 3.
The high pH of the extraction buffer inhibited endogenous folate conjugase
activity, and the sodium ascorbate prevented oxidative degradation of the folates.