counterstain


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counterstain

[′kau̇nt·ər‚stān]
(biology)
A second stain applied to a biological specimen to color elements other than those demonstrated by the principal stain.
References in periodicals archive ?
One method, known as alkaline phosphatase-anti-alkaline phosphatase, involves application of rabbit anti-mouse antibody and alkaline phosphatase-anti-alkaline phosphatase complex (immunoalkaline phosphatase) after incubation with PG-M3, with a hematoxylin counterstain.
Histological sections were cut at 5[mu]m and stained by hematoxylin followed by eosin counterstain (Presnell & Schreibman 1997).
We then applied counterstain solution (VECTASHIELD[R] Mounting Medium; Vector Laboratories) to the target areas and analyzed the slides with a fluorescence microscope equipped with a dual-bandpass filter set, as well as with single-bandpass filter sets.
Antibody binding was visualized with an avid-in-biotin-peroxidase kit (VEGTASTAIN Elite ABC Kit; Vector Laboratories, Burlingame, CA) with diaminobenzidine as the chromagen and hematoxylin as a nuclear counterstain.
The use of propidium iodide as a counterstain allowed the visualization and identification of chromosome pairs (Fig.
We used the avidin-biotin-peroxidase complex method with Harris hematoxylin counterstain.
All coverslips were mounted with mounting medium containing a proper dilution of 4',6-diamidino-2-phenylindole (DAP1) to counterstain DNA in the nuclei.
Slides were stained with Harris hematoxylin followed-by eosin counterstain (Presnell & Schreibman 1997).
Pontamine sky blue: a counterstain for background autofluorescence in fluorescence and immunofluorescence histochemistry.
The intensity of the bands in the RE treatments seems to be related to the type of counterstain used (e.