counterstain

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counterstain

[′kau̇nt·ər‚stān]
(biology)
A second stain applied to a biological specimen to color elements other than those demonstrated by the principal stain.
References in periodicals archive ?
Paraffin-embedded sections of rat mammary glands from each treatment group were stained with 3,3'-diaminobenzidine (DAB) using antibodies against Ki-67 and were counterstained with hematoxylin.
Tissues stained by using the dIHC protocol were not counterstained.
The reaction was terminated by washing in TPBS three times, 5 min each time, and then the embryos were counterstained with DAPI, rinsed with TPBS, and sealed with coverslips.
Sections were now counterstained with 90% ethanolic extract of Z.
The specimens were counterstained by immersion in hematoxylin.
Sections were counterstained in Mayer's haematoxylin [18] for 9 minutes, destained in acid alcohol for 10 seconds, blued in Scott's tap water substitute for 2 minutes, counterstained in eosin for 2 minutes, and dehydrated through graded alcohols to xylol.
2]), Nuclei were counterstained with hematoxylin, Slides were subsequently dehydrated following a standard procedure and sealed with coverslips, In order to minimize interassay variation, positive and negative control samples were included in each run, The positive control sample was lung cancer tissue, The negative control was prepared by substituting non-immune serum for the primary antibody; no detectable staining was evident, Because the nuclear translocation of ERK may be necessary for ERK-activated transcription, strong nuclear staining was considered positive for p-ERK,
The sections were then colorized with diaminobenzidine/hydrogen peroxide and counterstained with Harris modified hematoxylin.
005% hydrogen peroxide to visualize the reaction products finally, these sections were counterstained with hematoxylin.
The nuclei of the cells were counterstained with 1 mg/L 4',6-diamidino-2-phenylindole.
After hybridization, the slides were washed in first post hybridization wash buffer at 73[degrees]C for 2 min, and then in second post hybridization wash buffer at room temperature for 1 min and counterstained with DAPI.