The slides were washed with PBS,
counterstained with Wrights stain.
Paraffin-embedded sections of rat mammary glands from each treatment group were stained with 3,3'-diaminobenzidine (DAB) using antibodies against Ki-67 and were
counterstained with hematoxylin.
Tissues stained by using the dIHC protocol were not
counterstained.
The reaction was terminated by washing in TPBS three times, 5 min each time, and then the embryos were
counterstained with DAPI, rinsed with TPBS, and sealed with coverslips.
Sections were now
counterstained with 90% ethanolic extract of Z.
The specimens were
counterstained by immersion in hematoxylin.
Sections were
counterstained in Mayer's haematoxylin [18] for 9 minutes, destained in acid alcohol for 10 seconds, blued in Scott's tap water substitute for 2 minutes,
counterstained in eosin for 2 minutes, and dehydrated through graded alcohols to xylol.
2]), Nuclei were
counterstained with hematoxylin, Slides were subsequently dehydrated following a standard procedure and sealed with coverslips, In order to minimize interassay variation, positive and negative control samples were included in each run, The positive control sample was lung cancer tissue, The negative control was prepared by substituting non-immune serum for the primary antibody; no detectable staining was evident, Because the nuclear translocation of ERK may be necessary for ERK-activated transcription, strong nuclear staining was considered positive for p-ERK,
The sections were then colorized with diaminobenzidine/hydrogen peroxide and
counterstained with Harris modified hematoxylin.
005% hydrogen peroxide to visualize the reaction products finally, these sections were
counterstained with hematoxylin.
The nuclei of the cells were
counterstained with 1 mg/L 4',6-diamidino-2-phenylindole.
After hybridization, the slides were washed in first post hybridization wash buffer at 73[degrees]C for 2 min, and then in second post hybridization wash buffer at room temperature for 1 min and
counterstained with DAPI.
