counterstain

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counterstain

[′kau̇nt·ər‚stān]
(biology)
A second stain applied to a biological specimen to color elements other than those demonstrated by the principal stain.
References in periodicals archive ?
The sections were counterstained with hematoxylin and dehydrated through graded alcohols.
2005), the slides hybridized with the PNA probes were counterstained with propidium iodide, and the chromosomes bearing telomere FITC signals were observed and photographed with a confocal laser-scanning microscope (LSM510; Carl Zeiss, Jena, Germany).
2]), counterstained with haematoxylin solution (Sigma Aldrich, USA), dehydrated, cleared, and cover slipped.
The slides were washed again, counterstained with hematoxylin, and dehydrated with a graded alcohol series.
After immunoreaction, sections were counterstained with methyl green for nerve fibre studies, and Harris hematoxyline for neuroendocrine cell studies.
Samples were counterstained with Harris' hematoxylin (Biopur, Rosario, Argentina) for BrdU labeling or with Mayer's hematoxylin for CK8, p63, and RMCP-I detection, and mounted with permanent mounting medium (PMyR, Buenos Aires, Argentina).
All slides were stained simultaneously with the Schiff reagent and counterstained with 0.
The nuclei were lightly counterstained with Mayer hematoxylin solution.
Control slides were run simultaneously during each experiment and all the slides counterstained with methyl green (anti-apoptotic stain).
After 48 h incubation with the substances under test the apoptotic cells were stained with YO-PRO-1[R] dye (Invitrogen) and counterstained with Hoechst 33342 dye (Invitrogen).
Sections were washed 3 times and counterstained in the nuclear stain 4'-6-diamidino-2-phenylindole solution.
Sections were counterstained with haematoxylin, and then mounted with an aqueous mounting medium (DAKO Faramount Mounting Medium, Aqueous).