deamidation


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deamidation

[dē‚am·ə′dā·shən]
(organic chemistry)
Removal of the amido group from a molecule.
References in periodicals archive ?
We observed no such deamidation of proteins in hemolyzed plasma over a 4-h time period, but to prevent any possible ammonia production as a result of deamidation of the cellular proteins, ammonia samples should be centrifuged immediately and the EDTA plasma separated from cells, sealed, and stored at 4-8 [degrees]C, not frozen.
94 Da), but would occur if there were partial (60%) deamidation of [Asn.
Deamidation increased the number of positive sera among the treated and nontreated groups of CD patients, in particular for peptide-2 for both isotypes and for peptide-1 only for the IgG isotype.
Deamidation of glutamate by increased plasma [gamma]-glutamyltransferase is a source of rapid ammonia formation in blood and plasma specimens.
One possible explanation for these findings is that a C [right arrow] A mutation occurs in codon 50 of the [alpha]-globin gene, which causes the substitution of His for Asn, and that the new [alpha]50 Asn residue subsequently undergoes a posttranslational partial deamidation to Asp.
During its 18-day circulatory half-life, the mature molecule is subjected to a barrage of additional covalent modifications, including proteolytic cleavage, oxidation, and deamidation, and the covalent attachment of a variety of endogenous (e.
Deamidation of asparagine and glutamine and oxidation of methionine were selected for the search parameters as variable modification, and carbamidomethyl modification of cysteine was used as a fixed modification.
03 Da; trypsin as enzyme; up to one missed cleavage allowed; carbamidomethylation as a fixed modification; deamidation and oxidation as variable modifications.
In a second poster presentation entitled "Imaging Capillary Electrophoresis Discriminates Slow from Fast Asparagine Deamidation that may Predict Potency for Recombinant Protective Antigen Anthrax Vaccines," Dr.
The conversion of protein amide groups to carboxyl groups by deamidation improves solubility and other physical properties of proteins under mildly acidic conditions.
Deamidation of gliadin epitopes by transglutaminase enables them to be presented with high affinity by antigen-presenting cells to helper T cells and then B cells, resulting in antibody production against gliadin, transglutaminase 2, and gliadin-transglutaminase complex.
Irreversible protein denaturation can also be explained as resulting from deamidation of aspartic acid (As) and glycine (Gln) residues, cleavage of peptide bonds at As residues, destruction of cysteine (Cys) and cystine residues, and aggregation [22, 26].