To achieve the same separation reported for large size gel, two mini-gels with different ranges of denaturant
concentrations were used.
1981-2008: Data in thousand barrels for petroleum denaturant
in fuel ethanol produced are estimated as 2% of fuel ethanol production; these data are converted to Btu by multiplying by 4.
are typically used in automotive and industrial water- washed paint spray booths to denature and coagulate paint overspray making removal and waste treatment easier.
gel electrophoresis as a rapid screening technique for p53 mutations.
See Alternative-Fuel Vehicle, Denaturant
, E85, Ethanol, Fuel Ethanol Minus Denaturant
, and Oxygenates.
capillary electrophoresis (CDCE): a high resolution approach to mutational analysis.
BESS is considerably less labor-intensive than genomic sequencing and several other screening methods, such as constant denaturant
gel electrophoresis, denaturing gradient-gel electrophoresis, and in vitro transcription/ translation assays (3-5).
A narrow- or broad-range denaturant
gradient parallel to the direction of electrophoresis was required (15).
During the assay, sample is first mixed with denaturant
and extractant reagents.
For the technique to be successful, different conditions must be found and optimized because the slopes of the denaturant
gradient and running times vary for every DNA region to be analyzed; in addition, the long running time needed to resolve hetero- and homoduplexes often produces smears, affecting detectability.
The use of several denaturants
for SSCP has been reported in the literature; each report suggests better results with one class of denaturant
compared with another.
Omission of the urea denaturant
resulted in a 4% increase of our target tHcy value for the control sample, but the chromatographic profile without urea was less noisy and the assay variation (within-run CV) was correspondingly decreased from 4.