denature

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denature

[dē′nā·chər]
(chemistry)
To change a protein by heating it or treating it with alkali or acid so that the original properties such as solubility are changed as a result of the protein's molecular structure being changed in some way.
To add a denaturant, such as methyl alcohol, to grain alcohol to make the grain alcohol poisonous and unfit for human consumption.
References in periodicals archive ?
Qualitatively, the structure of these hybrids as compared to the structure of homoduplexes has shape-distorting mismatch(es); thus, they melt under milder denaturing conditions and have different rates of migration on the denaturing gradient gel.
Conventional PCR conditions were a single hot-start cycle at 95[degrees]C for 5 min, followed by 45 cycles of denaturing at 94[degrees]C for 45 s, annealing at 54[degrees]C for 45 s, and extension at 72[degrees]C for 45 s.
We now propose a simpler, rapid mutational approach based on denaturing HPLC (DHPLC) (4), with which we were able to confirm all of the nucleotide variations described in our first report (1) and to detect 5 other mutations in 7 of 15 unrelated Italian patients with a clinical suspicion of ATR-X syndrome.
The PCR products were loaded onto a 6% (wt/vol) polyacrylamide gel containing a denaturing gradient of urea and formamide.
The study results confirm that denaturing the mold spores with a dilute chlorine bleach solution appears to be the most effective and efficient way to reduce mold allergen on hard surfaces.
KEY WORDS: exclusive PCR; denaturing gradient gel electrophoresis: cry4 gene; Bacillus thuringiensis.
But their underlying structure is linear, and that makes them susceptible to unfurling, or denaturing.
EPIEN's products employ a patented chemical denaturing system to more effectively cleanse wound tissue of bacteria and cell debris while reducing the patient's pain and inflammation.
To rationalise the Community system in relation to the denaturing of alcohol and in agreement with the European action plan to strengthen the fight against tax fraud and tax evasion, JRC will finalise the harmonisation of completely denatured alcohol and initiate actions on partly denatured alcohol on specific sectors exempted under 27(1)(b) which MSs deem to be priorities: a) screenwash, de-icer, thinner, anti-freeze and solvents; and b) cosmetics, perfumes and hygienic products
Mutation detection sensitivity obtained with endoVII digestion was found to be similar to that for denaturing HPLC and direct sequencing (13).
Denaturing polyacrylamide gel materials were obtained from National Diagnostics (Atlanta, GA).
Pediococcus, Leuconostoc and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis.