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denature |
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denature [dē′nā·chər] (chemistry) To change a protein by heating it or treating it with alkali or acid so that the original properties such as solubility are changed as a result of the protein's molecular structure being changed in some way. To add a denaturant, such as methyl alcohol, to grain alcohol to make the grain alcohol poisonous and unfit for human consumption. How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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| Real-time PCR conditions were a single hot-start cycle at 95[degrees]C for 30 s, followed by 55 cycles of denaturing at 94[degrees]C for 10 s, annealing at 58[degrees]C for 6 s (for Bartonella) or at 60[degrees]C for 6 sec (for the 4 Bartonella species), and final extension at 72[degrees]C for 10 s. Denaturing polyacrylamide gel materials were obtained from National Diagnostics (Atlanta, GA). According to the government's announcement the test is a "more accurate genetic testing method, denaturing high performance liquid chromatography, or DHPLC screening. |
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