Qualitatively, the structure of these hybrids as compared to the structure of homoduplexes has shape-distorting mismatch(es); thus, they melt under milder denaturing
conditions and have different rates of migration on the denaturing
Conventional PCR conditions were a single hot-start cycle at 95[degrees]C for 5 min, followed by 45 cycles of denaturing
at 94[degrees]C for 45 s, annealing at 54[degrees]C for 45 s, and extension at 72[degrees]C for 45 s.
We now propose a simpler, rapid mutational approach based on denaturing
HPLC (DHPLC) (4), with which we were able to confirm all of the nucleotide variations described in our first report (1) and to detect 5 other mutations in 7 of 15 unrelated Italian patients with a clinical suspicion of ATR-X syndrome.
The PCR products were loaded onto a 6% (wt/vol) polyacrylamide gel containing a denaturing
gradient of urea and formamide.
The study results confirm that denaturing
the mold spores with a dilute chlorine bleach solution appears to be the most effective and efficient way to reduce mold allergen on hard surfaces.
KEY WORDS: exclusive PCR; denaturing
gradient gel electrophoresis: cry4 gene; Bacillus thuringiensis.
But their underlying structure is linear, and that makes them susceptible to unfurling, or denaturing
EPIEN's products employ a patented chemical denaturing
system to more effectively cleanse wound tissue of bacteria and cell debris while reducing the patient's pain and inflammation.
To rationalise the Community system in relation to the denaturing
of alcohol and in agreement with the European action plan to strengthen the fight against tax fraud and tax evasion, JRC will finalise the harmonisation of completely denatured alcohol and initiate actions on partly denatured alcohol on specific sectors exempted under 27(1)(b) which MSs deem to be priorities: a) screenwash, de-icer, thinner, anti-freeze and solvents; and b) cosmetics, perfumes and hygienic products
Mutation detection sensitivity obtained with endoVII digestion was found to be similar to that for denaturing
HPLC and direct sequencing (13).
polyacrylamide gel materials were obtained from National Diagnostics (Atlanta, GA).
Pediococcus, Leuconostoc and Weissella species in human feces by using group-specific PCR primers and denaturing
gradient gel electrophoresis.