deoxyribonucleotide


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Related to deoxyribonucleotide: Deoxyribonucleotide triphosphate

deoxyribonucleotide

[dē¦äk·sē‚rī·bō′nü·klē·ə‚tīd]
(biochemistry)
A nucleotide that contains deoxyribose and is a constituent of deoxyribonucleic acid.
References in periodicals archive ?
1 Cycle Sequencing Kit (Applied Biosystems, USA), comprising DNA polymerase, a mixture of deoxyribonucleotide triphosphates, and fluorochrome labeled dideoxynucleotide triphosphate.
4] Nonstandard abbreviations: qPCR, quantitative PCR; Cq, quantitation cycle; HRM, high-resolution melting; dNTP, deoxyribonucleotide triphosphate.
5 mM magnesium chloride (MgCl2), 200 [micro]M of each deoxyribonucleotide triphosphates (dNTP), 20 pmol/L of each set of specific primers (metabion international AG, Martinsried, Germany), 1 unit Taq DNA polymerase (MBI Fermentas, Amherst, NY, USA), and 0.
2 [micro]M deoxyribonucleotide triphosphate mix (Promega, Madison, WI, USA), 3 [micro]M Mg[Cl.
Polymerase chain reaction (PCR) was performed using primers and deoxyribonucleotide precursors provided by manufacturer and subsequent hybridization was done using the Twin-Cubator (Hain Lifescience) according to manufacturer's recommendations.
2] (Fermentase, Burlington; Canada), 50 mM KCl (Fermentase, Burlington; Canada), 10 mM deoxyribonucleotide triphosphate (dNTP) mix, 5 U/[micro]l Taq polymerase (Fermentase, Burlington; Canada), 5 pM of each primer (Bioneer; Korea), 500 ng DNA (Genomic; Korea) and sterile distillated water.
2 mM from each deoxyribonucleotide and 2 [micro]M from each primer.
We purchased the Superscript II Reverse Transcriptase (RT) kit, deoxyribonucleotide triphosphate (dNTPs), and Taq polymerase from Invitrogen (Karlsruhe, Germany); restriction enzymes from New England Biolabs (Frankfurt, Germany); random primers from Roche (Mannheim, Germany); and RNAse inhibitor from Promega (Mannheim, Germany).
Differential polymerase chain reaction (PCR) was performed to assess the GSTM1 polymorphism: 268 base pair (bp) ([beta]-globin) and 215 bp (GSTM1) PCR products were amplified using 30 pmol of primers for the GSTM1 gene and 10 pmol primers for the [beta]-globin gene in a 25 [micro]l reaction (200 [micro]M of each deoxyribonucleotide (dNTP), 3.
Glutathione possess various activities such as antioxidant defense, detoxification of electrophilic xenobiotics, and modulation of redox regulated, signal transduction, regulation of cell proliferation, synthesis of deoxyribonucleotide synthesis, regulation of immune responses, and regulation of leukotriene and prostaglandin metabolism [1, 6].
The functions of the skin such as cell replacement capability, physical, chemical, mechanical, microbial and thermal barrier, excretion of waste products, perception, immunomodulation, wound healing, sebum and sweat production, vitamin D synthesis, and capacity to repair deoxyribonucleotide (DNA),decreases with aging4.