However, pisiferic acid and oleic acid did not dephosphorylate
phospho-Ser112 Bad so much under the same conditions as for apoptosis induction (Figs.
In addition, we measured the total activity of the substrate by counting 20 [micro]L in a Beckman scintillation counter on the day of the assay, by measuring the spontaneous release via incubation of the substrate without lysate, and the capacity of AP (20 U) to dephosphorylate
the substrate (maximum dephosphorylation).
Samples were evaluated for their ability to dephosphorylate
1] is also able to dephosphorylate
a negatively regulating phosphorylation site in ZAP70 tyrosine-kinase (7).
G2 arrest ensues due to the inability of Cdc25c to dephosphorylate
inhibitory phosphorylation sites on Cdkl (Bartek and Lukas 2003).
However, the P component of phytate (282 g/kg) is only partially available to pigs because the endogenous phytase activity in the small intestinal mucosa is insufficient to effectively dephosphorylate
Protein phosphatases (PP1 and PP2B) were able to dephosphorylate
the PKC[alpha] phosphorylated N-DAT; however, none of the tested protein phosphatases dephosphorylated the ERK1 phosphorylated N-DAT.
Once the peptide reaches a critical density in stationery growth ph ase, it is sensed by VncS, which acts as a phosphatase to dephosphorylate
the response regulator, VncR.
The "on" state lasts as long as the phosphatase that can dephosphorylate
the enzyme is not present.
DUSP1, also known as MALIK phosphatase 1 (MKP1), can dephosphorylate
p38, which is considered to be an effective anti-inflammatory kinase that is controlled by GR.
Several members can efficiently dephosphorylate
p38[alpha] and p38[beta] (36-38); however, p38[gamma] and p38[delta] are resistant to all known MAPK phosphatase family members.
The reactions were carried out for 30 cycles at 96[degrees]C for 10 sec, 50[degrees]C for 5 sec and 60[degrees]C for 30 see in a PCR 9700 instrument (Applied Biosystems, Foster City, USA) and digested with Shrimp alkaline phosphatase to dephosphorylate
unincorporated fluorescent ddNTPs.