diaphorase


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diaphorase

[dī′af·ə‚rās]
(biochemistry)
Mitochondrial flavoprotein enzymes which catalyze the reduction of dyes, such as methylene blue, by reduced pyridine nucleotides such as reduced diphosphopyridine nucleotide.
References in periodicals archive ?
5 units/ml hexokinase, 16 units/ml G6PDH, and 1 unit/ml diaphorase, the mixture was incubated for 90 min.
The NADPH diaphorase assay was performed as described in Bishop and Brandhorst (2001) using pre-weighed vials of NADPH (Sigma.
For histochemical staining with NADPH diaphorase, the tissue specimens were incubated in 1 mg/mL [Beta]-NADPH (Sigma, Dorset, United Kingdom), 0.
Subsequently, diaphorase catalyzes the reduction of resazurin to the fluorescent compound resorufin.
We analyzed seven different enzyme systems: aconitate hydratase (Acn), diaphorase (Dia), isocitrate dehydrogenase (Idh), malate dehydrogenase (Mdh), phosphoglucoisomerase (Pgi), phosphoglucomutase, (Pgm) and Triose-phosphate isomerase (Tpi).
NOS activity, as detected by NADPH diaphorase activity (5), is present throughout larval development in Ilyanassa and is highest in metamorphically competent larvae, but it decreases dramatically once metamorphosis begins (6).
The HemoCue blood glucose analyzer is a bedside or near-patient analyzer with dedicated cuvettes; glucose dehydrogenase, mutarotase, and diaphorase act on the sample in connection with a color-generating reagent.
NO has also been identified in larvae of several invertebrates through the use of NADPH diaphorase (NADPHd) histochemistry (Lin and Leise, 1996b; Meleshkevitch et al.
Begleiter A, Leith MK, Curphey TJ, Doherty GP (1997) Induction of DT diaphorase in cancer chemoprevention and chemotherapy.
in a cyclic redox reaction in the presence of alcohol dehydrogenase, diaphorase, and INT-violet (amplifier); and (c) the production of a formazan end product with [A.