PCR products were sequenced by Sangers dideoxy method
(Scigenome, India) and the sequences edited using Gene Tool lite version 1.
Therefore we used the automated Sanger Dideoxy method
to control plasmid gWizHBs nucleotide sequence with focus on the sequence encoding for the hepatitis B s antigen.
The sequencing was done by the Sanger dideoxy method
(14) and this was followed by the detection of the HCV sequence on Beckman Coulter CEQ8000 genetic analysis system (Fullerton, USA).
Nucleotide sequencing was performed by the dideoxy method
Nested deletions of this clone were prepared (Henikoff 1987), and overlapping fragments were twice sequenced by the dideoxy method
(Sanger 1977) from double-strand plasmid templates using M13 primers.
Three clones were sequenced 3 times in both orientations by the dideoxy method
, using M13 universal primers and ET Dynamic Terminator for MegaBACE (GE Healthcare, Fairfield, CT, USA).
PSE-1]-related genes of several of the samples were sequenced by the dideoxy method
by using fluorescent terminators and an automatic laser fluorescent DNA sequencer (Pharmacia Biotech, Uppsala, Sweden) (Table) (6).
The England1 genome was generated by using 80 PCR products sequenced by Sanger dideoxy methods
(17), and the high level of sequence identity between the England/Qatar/2012 and England1 sequences strongly validates the novel rapid sequencing methods described here.