Digitonin


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digitonin

[‚dij·ə′tō·nən]
(pharmacology)
C41H64O13 A glycoside derived from the purple foxglove plant (Digitalis purpurea); a white powder melting at 255-256°C; used as a medicine for cardiac conditions.

Digitonin

 

a steroid saponin with surface-active properties; it is used to separate enzymes. It causes mitochondria to decompose into protein complexes that participate in conjugated enzyme processes not observed in mixtures of isolated enzymes.

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The situation, however, was complicated because pirenzepine was indicated to show similar affinity for the two receptor preparations solubilized with digitonin from either rat brain or heart.
3-[(3-Cholamidopropyl)-dimethylammonio]-1-propanesulphonate (CHAPS) and 3-[(3-cholamidopropyl)-dimethylammonio]-2-hydroxy-1-propanesulphonate (CHAPSO) were from Calbiochem (Darmstadt, Germany); N-(2-hydroxyethyl)-piperazine-N'-(2-ethanesulphonic acid) (Hepes), tris(hydroxymethyl)aminomethane (Tris), ethylene glycol-bis([beta]-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA), 1,6-diphenyl-1,3,5-hexatriene (DPH), sodium dodecyl sulphate (SDS), phenylmethylsulphonyl fluoride (PMSF), aprotinin, benzamidine, digitonin, cholic acid, deoxycholic acid, n-dodecyl-[beta]-D-maltoside (DBM), and dodecylpoly[(ethylene-glycolether).
Collagenase, ethylenediaminetetraacetic acid (EDTA), ethylene glycol tetra-acetic acid (EGTA), sodium pyrophosphate, sodium orthovanadate, sodium fluoride, okadaic acid, 1% protease inhibitor cocktail, dithiothreitol, magnesium chloride, K-lactobionate, taurine, potassium phosphate, HEPES, digitonin, pyruvate, malic acid, glutamic acid, adenosine diphosphate (ADP), succinic acid, oligomycin, carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP), antibody to [beta]-actin (mouse), phenylephrine and acetylcholine, trypsin inhibitor, and cytochrome c were procured from SigmaAldrich (MO, USA).
Additionally, in threedrug combinations of lignans with the saponin digitonin and doxorubicin MDR reversal activity was even stronger enhanced.
The results were expressed as the percentage of ATP released relative to the total ATP present in cells lysed by means of digitonin (50 [micro]M) (16).
94] described that, in digitonin permeabilized cardiomyocytes from young hearts, induction of SR [Ca.
14]C]-radio-labeled VLCFA substrates and one of two available methods: either in intact human skin fibroblasts cultured in monolayer (5); or in isolated fibroblasts permeabilized with digitonin (6).
2])GMP, isobutylmethylxanthine (IBMX), and digitonin were from Fluka (Ronkonkoma, NY).
The first colorimetric assay for cholesterol in serum, reported by Grigaut in 1910, combined purification by digitonin with an acid reagent.