direct immunofluorescence


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direct immunofluorescence

[də′rekt ¦im·yə‚nō·flu̇r′es·əns]
(immunology)
The use of labeled reactant to reveal the presence of an unlabeled one.
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Summary of the utility of the individual testing modalities (a) Viral Characteristic DIF (b) ELISA (c) culture (d) PCR (e) Specificity 90% 100% 100% 100% Sensitivity 70% 85% 100% 100% Assay time Hours Hours Days 1 day (a) DIF, direct immunofluorescence assay; ELISA, enzyme-linked immunosorbent assay; PCR, polymerase chain reaction.
Rapid diagnosis of influenza A infection by direct immunofluorescence of nasopharyngeal aspirates in adults.
Direct immunofluorescence testing of lesional or perilesional skin reveals IgG and C3 complement at the lamina lucida.
Six of 10 lymph node biopsy specimens were positive in a direct immunofluorescence assay with monoclonal antibodies for B.
Specifically, Darier disease usually lacks an eosinophilic infiltrate, and pemphigus vularis and foliaceous display the characteristic IgG and C3 expression on direct immunofluorescence in a fishnet pattern between individual keratinocytes.
1,2) Direct immunofluorescence of the epithelium will demonstrate a linear and homogeneous pattern of IgG and C3 deposition in the basal membrane.
Specimens from these children tested negative for RSV, parainfluenza viruses (types 1-3), influenza A and B viruses, and adenovirus by direct immunofluorescence assay as well as human metapneumovirus and HCoV-NH by RT-PCR.
Direct immunofluorescence assays (DFAs) are commonly used in the clinical virology laboratory for the rapid detection of a wide variety of viral pathogens.
Direct immunofluorescence assay (IFA) with fluorescein isothiocyanate-conjugated anti-Francisella serum (BulBioNCIPD) was used to detect F.
Direct immunofluorescence test on a sample of the patient's serum was positive 7 days later.
Because of the infant's tachypnea, a nasopharyngeal aspirate (NPA) was obtained for human respiratory syncytial virus (RSV) antigen testing (Test Pack, Abbott Laboratories, Abbott Park, IL) and direct immunofluorescence assays (Bartels, Carlsbad, CA) for adenoviruses and parainfluenza viruses 1-3.
According to the records, approximately 50% of the 50 North American patients, which were confirmed by direct immunofluorescence, histopathology, or both, were Hispanic American.

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