stain

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stain

a dye or similar reagent, used to colour specimens for microscopic study

Stain (microbiology)

Any colored, organic compound, usually called dye, used to stain tissues, cells, cell components, or cell contents. The dye may be natural or synthetic. The object stained is called the substrate. The small size and transparency of microorganisms make them difficult to see even with the aid of a high-power microscope. Staining facilitates the observation of a substrate by introducing differences in optical density or in light absorption between the substrate and its surround or between different parts of the same substrate. In electron microscopy, and sometimes in light microscopy (as in the silver impregnation technique of staining flagella or capsules), staining is accomplished by depositing on the substrate ultraphotoscopic particles of a metal such as chromium or gold (the so-called shadowing process); or staining is done by treating the substrate with solutions of metallic compounds such as uranyl acetate or phosphotungstic acid. Stains may be classified according to their molecular structure. They may also be classified according to their chemical behavior into acid, basic, neutral, and indifferent. This classification is of more practical value to the biologist. See Medical bacteriology

Stain

A coloring liquid or dye for application to any porous material, most often wood; thinner than paint and readily absorbed by the wood so that the texture and grain of the wood is enhanced, and not concealed.

stain

[stān]
(materials)
A nonprotective coloring matter used on wood surfaces; imparts color without obscuring the wood grains.
Any colored, organic compound used to stain tissues, cells, cell components, cell contents, or other biological substrates for microscopic examination.

stain

1. A discoloration in the surface of wood, plastic, sealant, etc.
2. A colorant for enhancing wood grain during finishing.
References in periodicals archive ?
Few studies have investigated the value of Ki67 indices quantified by DIA on single IHC stains, and to our knowledge none have been performed on IHC double stains.
In conclusion, we have shown excellent discrimination between melanomas and nevi by automated image analysis of Ki67/MART1 double stains with diagnostic performances equal to the manually performed indices by real-time microscopy and a counting frame.
The authors thank the medical laboratory technicians Allan Thorsteinsson, Martin Nielsen, Lone Nielsen, and Helle Johnsen from the Department of Pathology, Vejle Hospital, for performing the immunohistochemical double stains.
Immunohistochemical double stains against Ki67/MART1 and HMB45/MITF: promising diagnostic tools in melanocytic lesions.
The double stain with MUC4/p53 is shown in Figure 4 as follows: Figure 4, A and B, both MUC4 and p53 immunoreactivity in pancreatic adenocarcinoma; Figure 4, C, pancreatic adenocarcinoma showing staining for p53 and no immunoreactivity with MUC4; and Figure 4, D, pancreatic adenocarcinoma showing staining for MUC4 and no immunoreactivity with p53.
When the novel MUC4/p53 double stain was used and a positive for either stain was considered a positive result, the sensitivity was 96% and the specificity was 73%.
The double immunohistochemical stain for MUC4/p53 was then used on 20 needle core biopsies of the pancreas (10 adenocarcinoma and 10 benign) to further demonstrate the clinical utility of this double stain.