using sol-gel immobilized stationary phase with UV absorbance detection.
The monolithic columns that arose in the early 1990s stemmed from an effort to tackle these performance obstacles, most notably within the field of capillary electrochromatography
The Journal of Chromatography A publishes papers on all aspects of separation science including chromatography, electrochromatography
, electrophoresis, hyphenated and other multi-dimensional techniques, sample preparation, as well as detection methods such as mass spectrometry.
The second edition continues with these principles but has been updated to include new chapters on sampling and sample preparation, capillary electrophoresis and capillary electrochromatography
(CEC), chromatography with mass spec detection, and industrial and governmental practices in regulated industries.
The reference also explores recombinant proteins and proteomics, DNA sequencing and oligonucleotide analysis, capillary electrochromatography
, simulated moving bed processes, ion-exchange chromatography, and thin-layer chromatography.
Edward Lai has made substantial contributions to the analytical chemistry of biochemical and environmental materials by using such novel techniques as nonasecond laser spectroscopy, surface plasmon resonance, time-of-flight spectrometry and electrochromatography
, resulting in new photochemical, electrochemical, optical, and molecular recognition phenomena.
This modestly priced hardcover volume provides an excellent introduction to the emerging technologies of capillary electrochromatography
(CEC) and pressurized flow capillary electrochromatography
Other instruments used for drug testing include capillary GC with mass selective detector, capillary electrochromatography
and GC/MS/MS ion trap systems.
He has implemented capillary electrophoresis, capillary gel electrophoresis, open tubular electrochromatography
, and miscellar electrokinetic capillary chromatography separation techniques with his lab-on-a-chip devices.
Further examples of topics presented include separation of alkaloids by countercurrent chromatography, application of capillary electrochromatography
to biopolymers and pharmaceuticals, concentration of dilute colloidal samples by field- flow fractionation, laser-induced fluorescence detection for capillary electrophoresis, mass transfer between phases, molecularly imprinted polymers for affinity chromatography, metal-ion enrichment by countercurrent chromatography, photodiode-array detection, rotation locular countercurrent chromatography, quantitation by external and internal standards, systematic selection of solvent systems for high speed countercurrent chromatography, and cold wall effects in thermal field-flow fractionation.
Separation of nucleosides using capillary electrochromatography
An area closely related to CE that has yet to reach its stride is capillary electrochromatography
(CEC), which combines features of CE with LC by using capillaries, packed with chromatographic materials.