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emission spectrum |
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emission spectrum: see spectrum spectrum, arrangement or display of light or other form of radiation separated according to wavelength, frequency, energy, or some other property. Beams of charged particles can be separated into a spectrum according to mass in a mass spectrometer (see mass ..... Click the link for more information. . |
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5) is performed on the emission spectrum from a reference solution with known concentration of fluorophore and the emission spectrum from a suspension of microspheres with immobilized FITC. It is widely accepted that describing a measured x-ray peak by a Gaussian distribution is a sufficient approximation to derive accurate x-ray intensities from an x-ray emission spectrum measured with an EDS. It has been found that emission spectrum matching between a reference solution and an analyte and normalization by the corresponding extinction coefficient are required for quantifying fluorescence signals using flow cytometers. |
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