exonuclease


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exonuclease

[¦ek·sō′nü·klē‚ās]
(biochemistry)
Any of a group of enzymes which catalyze hydrolysis of single nucleotide residues from the end of a deoxyribonucleic acid chain.
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Recombinant, untagged human Ape1, Escherichia coli exonuclease III (ExoIII), and E.
Detection of specific polymerase chain reaction product by utilizing the 5'--3' exonuclease activity of Thermus aquaticus DNA polymerase.
17,39,40) Pol [gamma] with diminished DNA polymerase activity and/or reduced exonuclease activity with weakened proofreading can lead to a significant decrease in mtDNA copy number, or multiple deletions in mtDNA, which in turn translates to defects in essential OXPHOS proteins, the functional consequences of which can be related to clinical outcome.
The PCR products are treated with Exonuclease I to digest any unincorporated primers and shrink alkaline phosphatase to remove the 5' phosphates of any nucleotides that are not incorporated.
Sequencing of the staphylococcal protein A gene (spa typing) and multilocus sequence typing (MLST) were performed as previously described (14,15), except that PCR products were enzymatically purified with exonuclease I (New England Biolabs) and shrimp alkaline phosphatase (Amersham Biosciences, Piscataway, NJ, USA) before spa sequencing.
For construction of the Gal4 DBD-PXR ligand binding domain (LBD) fusion plasmid (Gal-PXR), PCR was used to amplify LBD-specific fragments of rat PXR; exonuclease III-mediated subcloning (Li and Evans 1997) was used to clone the fragments between the EcoRI and BamHI sites of pCMX-Gal4.
anaphase backcross diakinesis segregate translocation mutation allele sequence phenotype locus oligonucleotide prophase chromomere nucleus meiosis pedigree replicon zygote genotype exonuclease cytogenetics codominance nucleolus Metric System decimeter quintal microgram dekagram teragram meter terameter megaliter milligram hectokilogram micrometer kilometer centiliter hectometer gram decimilligram kilogram nanometer liter microliter nanogram decigram gigagram centimeter gigameter kiloliter dekaliter picogram centigram megagram millimeter megameter deciliter centimilligram hectogram picometer dekameter picoliter teraliter
Both pathways can occur in parallel, because Taq polymerases are known to possess different levels of exonuclease activity (22) and may dissociate hairpin structures with only partial digestion of the 5' terminus (23).
There are several PCR product clean-up methods (eg, treatment with the mix of exonuclease I from Escherichia coli and thermosensitive alkaline phosphatase, the DNA binding on silica matrix, etc) available to minimize the loss of amplified DNA.
The enzyme has both five prime three prime DNA polymerase and exonuclease activities, is approximately 95kD by SDS-PAGE, and has no detectable endonuclease or three prime five prime exonuclease activities.
Two methods of DNA sequencing in development, Exonuclease and Strand sequencing, combine a protein nanopore with a processive enzyme, multiplexed on a silicon chip.
PCR products were purified with exonuclease I (New England Biolabs) and shrimp alkaline phosphatase (USB/Affymetrix) according to the manufacturers' instructions.