expression vector


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expression vector

[ik′spresh·ən ‚vek·tər]
(cell and molecular biology)
A cloning vector that promotes the expression of foreign gene inserts.
References in periodicals archive ?
Most of the cells in the stably transfected HIF-1 [alpha] RNAi expression vector pcDNA6.
After transfection of the Cas9-SH3RF2 gRNA expression vector into QM7 cells, GFP-positive cells were identified by FACS and a single cell was isolated followed by in vitro expansion.
Protein Expression and Purification- Rat nNOS cDNA was cloned into the recombinant expression vector pKLSLt and expressed in E.
The expression vector with Taq DNA polymerase gene, developed in the present study, will certainly expand more avenues for heat-tolerant genetic transformation in plants.
For the development of stable cell pools, CHO-K1 cells were transfected with the pLCIHC-Puro expression vector in duplicates.
After receiving of hpaA construction in PGH vector and confirmation by mentioned methods in material & method section, has been successfully sub-cloned into the expression vector pET21b.
This vp2 was purified and digested again with specified restriction enzymes and inserted into expression vector pPG612-HCE-PgsA-rrnBT1T2 (4954bp) to get pPG612-HCE-PgsA-vp2-rrnBT1T2 expression vector and also ligated T7g10 enhancer to pPG612-HCE-PgsA-vp2-rrnBT1T2 to get pPG612-HCE-T7g10-PgsA-vp2-rrnBT1T2 expression vector.
In this study, the VEGF gene was isolated from HeLa cells for the first time and the expression vector for [VEGF.
In order to clone in the expression vector plasmid pET-21a was digested with the same two restriction enzymes (NdeI and HindIII) and resolved on agarose gel.
2007) were co-transfected with Renilla luciferase expression vector (TK-RL), respectively.
The protein then was cloned into the pET-32a expression vector.
However, using VISA, short for versatile expression vector, which was developed at M.