Effect of different volumes of Glasgow minimum essential medium (GMEM) with 5% fetal calf
serum (FCS) was monitored on growth of BHK-21 cells in roller culture bottles.
For immunofluorescence studies, the OC exptants were fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 5% Triton X-100 in PBS with 10% fetal calf
Cells were cultured in DMEM containing 25 mmol/L glucose and 200 mL/L heat-inactivated fetal calf
serum and grown at 37 [degrees]C in air containing 5% [CO.
In the past, the islets have all been isolated with fetal calf
On the other side was a special mixture of fetal calf
serum that acted as an attractant to the neutrophils.
Belot and colleagues remind us that in vitro tests often still involve the use of fetal calf
serum or other products of animal origin.
Three types of maturation media (MM) supplemented with 1) 10% fetal calf
serum (FCS), 2) 6 mg/ml FAF-BSA or 3) 6 mg/ml FAF-BSA + 10.
On day 3 after sample inoculation, the antimicrobial drug-containing medium was removed and replaced with MEM without antimicrobial drug and supplemented with 5% fetal calf
serum (HyClone Laboratories, Inc.
HEK-293 cells (22) (ATCC number CRL-1573) were cultivated in DMEM supplemented with 100 mL/L fetal calf
serum, 10 kIU/L penicillin, and 100 mg/L streptomycin.
Briefly, we added the aliquots of the HPLC fractions (1-5 [micro]L) or HPLC-purified synthetic LTX-diol isomers in redistilled ethanol to cultured MCF-7 cells grown in phenol red-free Dulbecco's modified Eagle's medium (DMEM) containing 5% charcoal-stripped, sulfatase-treated fetal calf
HT1080 cells were grown in supplemented DME medium containing glutamine (1 mM), penicillin (100 IU/ml), streptomycin, (100 [micro]g/ml), and 10% heatinactivated fetal calf
The virus was propagated on monolayer of BHK-21 cell line in Roux flasks containing 100 ml of maintenance medium with 1 % fetal calf
serum (Muhammad et al.