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Related to fluorescence polarization immunoassay: Cloned Enzyme Donor Immunoassay


An assay that quantifies antigen or antibody by immunochemical means. The antigen can be a relatively simple substance such as a drug, or a complex one such as a protein or a virus. See Antibody, Antigen

The reactants are first mixed so that a varying quantity of one (A) is added to a constant amount of the other (B). The formation of an immune (antigen-antibody) complex is measured as a function of the varied reactant (A). The result is represented by a “standard curve” for reactant A. An unknown sample is tested by adding it to reactant B. The extent of the measured change is referred to the standard curve, and thereby is obtained the amount of reactant A which produces a comparable change. The amount is represented as the content of reactant A in the unknown sample. See Immunofluorescence, Immunology, Radioimmunoassay


A laboratory detection method that uses antibodies to react with specific substances.
References in periodicals archive ?
A significant apparent digoxin concentration was observed both in vitro and in vivo using the fluorescence polarization immunoassay, but no apparent digoxin concentration was observed using enzyme-linked chemiluminescent immunosorbent digoxin assay and turbidimetric assay.
3) It has been previously reported that Asian and American ginsengs and Ashwagandha (Indian ginseng) interfere with serum digoxin measurement by the fluorescence polarization immunoassay (FPIA).
The fluorescence polarization immunoassay kits for digoxin were purchased from the Abbott Laboratories (Abbott Park, Ill) and the assays were run on an FLx/TDx analyzer (Abbott).
20 * Measured by the fluorescence polarization immunoassay.
4] Nonstandard abbreviations: tHcy, total homocyst(e)ine; SBDF, ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate; GC-MS, gas chromatography-mass spectrometry; EIA, enzyme immunoassay; and FPIA, fluorescence polarization immunoassay.
The Abbott Laboratories AxSYM monoclonal fluorescence polarization immunoassay and TDx-m use the same proprietary antibody.
58), who stated that specific fluorescence polarization immunoassay and RIA methods provided valid results irrespective of their cross-reactivity.
Monoclonal fluorescence polarization immunoassay evaluated for monitoring cyclosporine in whole blood after kidney, heart, and liver transplantation.
Is the monoclonal fluorescence polarization immunoassay for cyclosporine specific?
Cyclosporine monitoring by fluorescence polarization immunoassay.
Methotrexate measurements in plasma: comparison of enzyme multiplied immunoassay technique, TDx fluorescence polarization immunoassay, and high pressure liquid chromatography.

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