fluorescent antibody technique definition of fluorescent antibody technique in the Free Online Encyclopedia.

immunofluorescence [¦im·yə·nō·flə′res·əns]

(immunology)

Fluorescence as the result of, or identifying, an immune response; a specifically stained antigen fluoresces in ultraviolet light and can thus be easily identified with a homologous antigen.

Immunofluorescence

A technique that uses a fluorochrome to indicate the occurrence of a specific antigen-antibody reaction. The fluorochrome labels either an antigen or an antibody. The labeled reactant is then used to detect the presence of the unlabeled reactant. The use of a labeled reactant (such as an antibody which both detects and indicates the antigen) to reveal the presence of an unlabeled one is termed direct immunofluorescence. The use of a labeled indicator antibody, which reacts with an unlabeled detector antibody that has previously reacted with an antigen, is termed indirect immunofluorescence. Substitution of a light meter for the human eye permits a quantitative measurement in immunofluorometry. See Immunoassay

Immunofluorescence

any set of methods of fluorescent analysis used in immunology, histochemistry, virology, bacteriology, mycology, and parasitology.

The combination of immunochemical reactions with fluorescence microscopy makes possible the detection of tissue and cellular antigens, including those involved in autoimmune diseases and in malignantly degenerated cells. The method is also useful in studying the patterns of antibody synthesis and in identifying the causative agents of many viral and microbial diseases. Specific antibodies are tagged with a fluorescent dye (for example, acridine orange) that will not alter their properties and then introduced to the specimen, so that only the parts of the specimen containing antigen will fluoresce. When the formation of antigen-antibody complexes is being investigated by immuno-fluorescence, the antibodies are tagged with a dye whose fluorescent properties will change when the antibodies combine with antigen.

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No references found			Antibody titers to LLAPs also were determined by the indirect fluorescent antibody technique. Legionella-like and other amoebal pathogens as agents of ... by de Carolis, Emidio / Emerging Infectious Diseases Hybridoma technology was combined with the fluorescein isothiocyanate (FITC) fluorescent antibody technique developed in the late 1950s. Clinical applications of flow cytometry by Scholes, Vernon E. / Medical Laboratory Observer			fluke Flukes flume flumed flunk fluoborate fluoborite fluocerite fluolite fluometuron fluophor fluor fluoranthene fluorapatite fluorene Fluorescein Fluorescence fluorescence analysis fluorescence digital imaging microscope fluorescence method fluorescence microscope fluorescence spectra fluorescence x-rays fluorescence yield fluorescent antibody fluorescent antibody technique fluorescent antibody test fluorescent dye fluorescent in situ hybridization Fluorescent Lamp fluorescent lighting fixture fluorescent minituft method fluorescent paint Fluorescent Penetrant Inspection fluorescent pigment fluorescent pigments fluorescent reflector lamp fluorescent screen fluorescent snaking fluorescent staining fluorescent strip fluorescent tube fluorescent U-lamp fluorescent-mercury lamp fluoridation fluoridation of water Fluoride Fluorides, Natural fluorimeter fluorinated ethylene propylene resin fluorination			Fluorescence Water Vapour Sensor fluorescence x-rays fluorescence yield Fluorescence Yield Near Edge Spectroscopy fluorescence-activated cell sorter (FACS) fluorescence-activated cell sorter (FACS) fluorescence-activated cell sorter (FACS) Fluorescence-Based Amplified Fragment Length Polymorphism Fluorescence-Based Single-Strand Conformation Polymorphism Fluorescence-Detected Linear Dichroism Fluorescence-Detected Magnetic Circular Dichroism Fluorescence-Microwave Double-Resonance Fluorescence-Quenching-Resolved Spectroscopy Fluorescences Fluorescences Fluorescences Fluorescense Fluorescense Fluorescense fluorescent fluorescent fluorescent fluorescent Fluorescent Activated Cell Sorter Fluorescent Amplified Fragment Length Polymorphism Fluorescent angiography fluorescent antibody fluorescent antibody fluorescent antibody fluorescent antibody fluorescent antibody technique fluorescent antibody test fluorescent antibody test fluorescent antibody test fluorescent antibody test fluorescent antibody test fluorescent antibody test Fluorescent Antibody Viral Neutralization Fluorescent Antibody Virus Neutralization Test Fluorescent Antinuclear Antibody fluorescent antinuclear antibody test Fluorescent Biprobe Hybridization fluorescent bone marker fluorescent bone marker Fluorescent bulb fluorescent bulbs Fluorescent Compound Fluorescent Compound Fluorescent Compound fluorescent crystals fluorescent crystals Fluorescent Damage Products of Lipid Peroxidation Fluorescent Derivative of a Fatty Acid Fluorescent Dissolved Organic Matter fluorescent dye fluorescent dye fluorescent dye Fluorescent dyes Fluorescent Fiber-Optic Immunosensor fluorescent fixture Fluorescent Focus-Forming Unit

Immunofluorescence (redirected from fluorescent antibody technique)

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Immunofluorescence
(redirected from fluorescent antibody technique)