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fluorimeter |
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Fure-2-loaded muscle strips were held horizontally in the organ chamber of a fluorimeter (CAF-110; Jasco, Tokyo, Japan) filled with KH solution. At the end of each annealing step, the fluorescent signal of each reaction was measured at a wavelength of 530 nm with the LightCycler fluorimeter. The measurements will be carried out using a conventional fluorimeter consisting of a monochromator with a CCD detector at the exit slit, and a source consisting of a cuvette with a illumination region parallel to the entrance slit of the monochromator. |
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