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Fluorochrome

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Fluorochrome 

a substance used in fluorescence, or luminescence, microscopy to study objects that do not possess a natural capacity to fluoresce. When fluorochromes are introduced into an organism, they are adsorbed by the cells, which consequently acquire the capacity to fluoresce. Fluorochromes may be dyes (auramine, coryphosphin), pigments and their derivatives (chlorophyll, porphyrin), or alkaloids (berberine). Fluorescence in microscopic objects stained with fluorochromes is brought about by ultraviolet, violet, or blue light. Fluorescence microscopy with the use of fluorochromes brings out structural details more clearly than does ordinary staining, especially in the case of biological specimens. Because the fluorescence microscopy method is highly sensitive, the fluorochrome concentration can be very low and consequently living organisms can be observed and their metabolic processes studied.

REFERENCES

Levshin, V. L. Fotoliuminestsentsiia zhidkikh i tverdykh veshchestv. Moscow-Leningrad, 1951.
Zelenin, A. V. Liuminestsentnaia tsitokhimiia nukleinovykh kislot. Moscow, 1967.
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The ScanScope FL acquires high-resolution multicolor digital images that can be archived permanently, eliminating concerns about fading of the fluorochromes on the glass slides over time.
Scanner for immunofluorescense samples by Medical Laboratory Observer
In the course of a CGH experiment, the patient's DNA and a reference DNA obtained from a healthy donor are labeled with different fluorochromes and cohybridized onto slides carrying metaphase spreads of another healthy donor.
An Integrative Approach for Analyzing the Interplay of Genetic and ... by Artur Muradyan and Vivien Boldt and Anne Steininger and Stephanie Stabentheiner and Katrin Tebel and / Archives of Pathology & Laboratory Medicine
Light Source Requirements The most basic requirement of a fluorescence microscopy light source is closely matching the excitation wavelength of the fluorochrome to achieve a high contrast image - that is, an image with a high signal-to-noise ratio.
LED LIGHT SOURCE A MAJOR ADVANCE IN FLUORESCENCE MICROSCOPY by Biotech Equipment Update
 
 
 
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