Results of modified one tube osmotic fragility test
(OF) and dichlorophenolindophenol precipitate (DCIP) test, hematological analyses for 12 participants.
A A peripheral blood osmotic fragility test has traditionally been considered important in the workup of hereditary spherocytosis (HS).
Unfortunately, many clinicians, especially those who have been practicing for a long time, continue to believe that the osmotic fragility test is the "gold standard" for HS and are reluctant to stop ordering it.
Screening for thalassemias and hemoglobinopathies was carried out by microscopic examination of a stained peripheral slide, osmotic fragility tests
, measurement of serum ferritin, cation exchange high performance liquid chromatography (HPLC) for Hb variants quantitation, alkaline and acid pH electrophoresis, sickle test, and DNA analysis when required.