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gel filtration |
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gel filtration [¦jel fil′trā·shən] (analytical chemistry) A type of column chromatography which separates molecules on the basis of size; higher-molecular-weight substances pass through the column first. Also known as molecular exclusion chromatography; molecular sieve chromatography. How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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After being washed with -20 column volumes, recombinant ApoC-I was refolded into the column, concentrated, loaded onto a gel filtration column (Superdex 75, HR 26/60), and eluted in 20 mM Tris buffer, 200 mM NaCl, pH 8. Vaccine research evaluates the potential of dendritic cells, T27K fractionated by gel filtration and anion exchange, and a recombinant Ag2/PRA106 + CSA chimeric fusion protein. 2,3 Gel filtration chromatography (GFC) of serum led to the identification of 3 major forms of prolactin immunoreactivity. |
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