Conclusions: These findings indicate that paeoniflorin might exert its therapeutic effects via upregulation of glyoxalase system and mitochondrial function.
However, the system that handles most of the cellular MG is the glyoxalase system, in which detoxification of MG is achieved by the sequential reaction of two enzymes, glyoxalase I and glyoxalase II, using the cofactor glutathione (Thornalley, 2003).
Glyoxalase I activity was measured using a modification from a previously published method (Thornalley and Tisdale, 1988).
Effect of paeoniflorin on glyoxalase system in MG-treated MC3T3-E1 cells
MG-mediated damage is countered by glutathione-dependent metabolism by glyoxalase I.
In conclusion, paeoniflorin reduced MG-induced MC3T3-E1 cell death by enhancing glyoxalase system and inhibition of the glycation mechanism including MG-derived oxidative stress and protein adducts formation.
Nevertheless, RAGE effects are tightly associated with glycoxidation, since its activation downregulates the expression of glyoxalase I, subsequently increasing concentrations of glyoxalase I substrates (e.
For example, several glyoxalase I and RAGE gene polymorphisms have been shown to be associated with various pathological states (e.