Screening: All subjects underwent peripheral venous blood sampling for a complete haemogram
, renal function tests, and thyroid profile.
Laboratory investigations including haemogram
, blood biochemistry with detailed liver functions (serum bilirubin, AST, ALT, alkaline phosphatase (ALP), serum total protein, serum albumin) were performed in all patients using standard laboratory procedures.
At the time of recruitment, at 0800 h, blood samples (10 ml) were drawn for the following investigations: haemogram
, renal and liver function tests, and pooled samples (two aliquots of similar volume at 20 min intervals) for serum prolactin, T3, T4, TSH, cortisol, LH, FSH and testosterone.
Blood samples (2 ml of EDTA blood from NESTROF test and 3 ml of non-ox lood for MSAFP) were collected from a subgroup of women with known last menstrual period (LMP) between 13-16 wk of gestation for estimation of maternal serum alpha-foetoprotein (MSAFP) and NESTROFT test (Naked Eye Single Tube Osmotic Fragility Test) along with routine haemogram
is the most frequent test done in any person seeking medical help.
World Health Organization (WHO) initiated an international QC programme in haemogram
in 1987 in which many international and national haematological laboratories from both developing and developed countries participated.