Physarum heavy meromyosin (HMM) was expressed with HC cDNA of Met 1-Lys 118, CaLc, and PLc, followed by similar purification procedures.
2007) Calcium inhibition of Physarum myosin as examined by the recombinant heavy meromyosin.
Squid mantle muscle contains endogenous metalloproteinases that selectively degrade myosin molecules into heavy meromyosin (HMM) and light meromyosin (LMM) (Yoshioka et al.
Yoshioka T, Kinoshita Y, Kato S, Cho Y, Konno K (2005) Preparation of heavy meromyosin from the autolyzed squid mantle homogenate.
Preparation of N-ethylmaleimide-modified heavy meromyosin
and its use as a functional probe of actomyosin-based motility.
Stepwise motion of an actin filament over a small number of heavy meromyosin
molecules is revealed in an in vitro motility assay.
In the Characean cell, cortical fibrils were confirmed to be composed of actin filament bundles by observation of the arrowhead structure formed by binding with skeletal muscle heavy meromyosin.
Estimation from slow sliding movement of actin over low densities of heavy meromyosin.