Histochemistry

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histochemistry

[¦hi·stō′kem·ə·strē]
(biochemistry)
A science that deals with the distribution and activities of chemical components in tissues.

Histochemistry

 

the division of histology that studies the chemical properties of animal and plant tissue.

Histochemistry seeks to elucidate the characteristics of metabolism in tissue cells and interstitial media. It studies changes in cell properties in the process of development and the connection between the work, metabolism, and renewal of mature cells and tissues. A basic principle of histochemical methodology is the bonding of a definite chemical component of cells with a stain or the formation of a stain in the process of the reaction. A number of methods (cytophotometry and luminescence and interference microscopy) are based on the physical properties of substances. Various histochemical methods determine the localization and quantity of many substances in tissue and their metabolism (tissue autoradiography), bonds with submicroscopic structure (electron histochemistry), and enzyme activity. Immunohistochemistry is a promising trend. The most precise histochemical methods, which permit investigation of cell structure, are called cytochemical methods.

The first special histochemical research was done by the French scientist F. Raspail (1825-34). Histochemistry began to develop intensively beginning in the 1940’s, when reliable methods appeared for determining proteins, nucleic acids, lipides, polysaccharides, and certain inorganic components in the cell. By means of histochemical methodology it has been possible, for instance, to show for the first time the connection of changes in the quantity of RNA with the synthesis of protein and the constancy of DNA content in the chromosome bank.

REFERENCES

Pearse, E. Gistokhimiia: Teoreticheskaia i prikladnaia. Moscow, 1962. (Translated from English.)
Burstone, M. Gistokhimiia fermentov. Moscow, 1965. (Translated from English.)

V. IA. BRODSKII

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Semiquantitative histochemical evaluations were also performed in the gonadal tissue to determine whether the levels of carbohydrates and lipids in the oocytes correlated with the degree of gonad maturation and/or oocyte diameter.
The data showing degree of histochemical reaction are presented in table 4.
Lectin histochemical analysis showed that sialic acid [alpha] 2,6-Gal (human-like influenza virus receptor) was absent from epithelial surface of small and large intestines and was found only in lamina propria cells.
The structure of the surface wall of these gametophytes, including the lipid materials, was examined with histochemical techniques and electron microscopy by Whittier and Peterson (1995).
The activity of NOS was first determined by use of enzyme histochemical assays and after the development of antibodies by immunohistochemical methods, although the development and use of antibodies proved to be challenging (2).
Numerous tools and techniques have been used to study wrinkles--histological, ultrastructural, histochemical, epidemiological, biophysical and mathematical.
of Newcastle, Australia) describes how microwave irradiation is being used in fixation, tissue preparation, and histochemical and immuno-histological staining as an alternative to the conventional dyes and other methods that are either environmentally damaging or very slow, and sometimes even both.
Morphological, histochemical and morphometric study of the myotomal muscle tissue of the pacu (Piaractus mesopotamicus Holmberg 1887: Serrasalminae, Characidae, Teleostei).
Distribution of zinc in the Gills of experimentally exposed catfish : Histological and Histochemical study.
A part of these investigations dealing with the malfunctioning of infected placenta at histological, scanning electron microscope, biochemical and histochemical localisation of hydrolases has already been published (15-18) and the present communication deals with the histochemical investigations on some metabolic marker oxidoreductases, the steroidogenic enzymes of malaria infected early gestational (2-2 1/2 months) monkey placenta.