holoenzyme


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Related to holoenzyme: apoenzyme

holoenzyme

[¦häl·ō′en‚zīm]
(biochemistry)
A complex, fully active enzyme, containing an apoenzyme and a coenzyme.
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19) Additionally, biochemical analysis of a point mutation in POLG2 (G451E) revealed that this mutation impaired subunit interaction with pol [gamma]A, reducing the functional capacity of the holoenzyme because the mutant pol [gamma]B failed to enhance DNA binding and processivity of the holoenzyme.
66 scFv and biotin holoenzyme synthetase was induced by the addition of isopropyl [beta]-n-thiogalactopyranoside to 250 [micro]mol/L and L-arabinose to 1.
coli through a reaction catalyzed by the enzyme biotin holoenzyme synthetase (BirA).
1] Nonstandard abbreviations: HAMA, human anti-mouse antibody; CEA, carcinoembryonic antigen; Mab, monoclonal antibody; scFv, single-chain fragment of variation; BSA, bovine serum albumin; PBS, phosphate-buffered saline; BirA, biofin holoenzyme synthetase; and BCCP, biofin carboxyl carrier protein.
But only after the cell convenes this holoenzyme does RNA polymerase proceed to the specific transcription site, Young and Koleske report in the March 31 NATURE.
The discovery of a holoenzyme clearly has major implications in understanding the mechanism of transcription," says Michael H.
Kornberg and his colleagues at Stanford University School of Medicine suggests that other proteins link with RNA polymerase and form a different sort of holoenzyme that then starts transcribing DNA.
The composition of the holoenzyme is still up in the air as far as I am concerned" says Sayre.
The reconstituted holoenzyme was then used in the PLP enzymatic assay with HCY as described above.
PLP is removed from the rHCYase holoenzyme by incubation with hydroxylamine to obtain apo-rHCYase.