housekeeping gene


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housekeeping gene

[′hau̇s‚kēp·iŋ ‚jēn]
(genetics)
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Probes for several external controls and housekeeping genes were designed with use of the Motorola Life Sciences probe-selection algorithm according to properties such as melting temperature, GC content, position of RT-PCR products, and free energy of probe-target hybrid.
Standard housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a calibrator.
A similar pattern of hsp 60 basal expression was also shown for the UROtsa cell line, with hsp 60 mRNA expression being below that of the housekeeping gene (Rossi et al.
0-fold range in expression seen in the housekeeping genes (average plus 2 standard deviations).
We evaluated 5 housekeeping genes (housekeeping gene selection set; Roche): [delta]-aminolevulinate synthase 1 (ALAS1), [[beta].
Characterization of housekeeping genes in zebrafish: male-female differences and effects of tissue type, developmental stage and chemical treatment.
18S rRNA was chosen as a housekeeping gene based on our previous studies on hemic neoplasia in softshell clams (Siah et al.
2004) based on the calculation of a housekeeping gene index from among several housekeeping genes (AldolaseA, [beta]-actin, GAPDH).
Expression level of ACT1 gene, which is a housekeeping gene, was determined in each set of experiment (data not shown).
org/szooepidemicus/) based on the allelic profile of 7 housekeeping gene fragments.