hybridization probe


Also found in: Dictionary, Medical, Wikipedia.
Related to hybridization probe: DNA probe, nucleic acid hybridisation, nucleic acid probes, Nucleic acid hybridization

hybridization probe

[‚hī·brəd·ə′zā·shən ′prōb]
(cell and molecular biology)
A small molecule of deoxyribonucleic acid or ribonucleic acid that is radioactively labeled and used to identify complementary nucleic acid sequences by hybridization.
References in periodicals archive ?
The hybridization probes for SCA2 and SCA3 were designed against the conserved region located by using the clustalW alignment tool.
Alternate approaches to probe based real-time PCR include the use of molecular beacons, Scorpion probes, and hybridization probes.
Finally, we investigated the effects of hybridization probe number, hybridization time, and template DNA quantity on the discrimination power of the MLPA/ rtPCR method.
V600E by snapback analysis but not by hybridization probe melting were confirmed by sequencing the snapback primer-amplified product.
Using the nearest neighbor model for the estimation of matched and mismatched hybridization probe melting points and selection of optimal probes on the light-cycler.
The hybridization probes contain a 5' MGB and fluorophore with a 3' quencher.
Primers and hybridization probe for TBP were as follows: gTgTCCACggTgAATCTTgg (ga1TBP F, 57.
Because melting curve analysis with hybridization probe technology is routinely used in our laboratory (3), we conducted a study to implement IVS8 genotyping and confirm its reliability.
Application of a thermodynamic nearest-neighbor model to estimate nucleic acid stability and optimize probe design: prediction of melting points of multiple mutations of apolipoprotein B-3500 and factor V with a hybridization probe genotyping assay on the LightCycler.
Multicenter evaluation of a Candida albicans peptide nucleic acid fluorescent in situ hybridization probe for characterization of yeast isolates from blood cultures.
For each RET exon, sample DNA (~50 ng total) was amplified with the LightCycler[R] FastStart DNA Master Hybridization Probe Kit (Roche Diagnostics Corp.