This was comparable to that of hygromycin (HYG) but higher than the required amount of nourseothricin (NAT) and caspofungin (CAS).
In contrast, inclusion of sorbitol had no effect on the hygromycin B MIC.
Hygromycin B inhibition of protein synthesis and ribosome biogenesis in Escherichia coli.
7 (a) CTR = no compound control; ECH = Echinacea extract; Alk2b to AIkl2 = alkamides (see Table 1); CAS = caspofungin; HYG = hygromycin B; NAT = nourseothricin.
A toxicity test was used to determine the levels of hygromycin B needed to effectively halt wild-type K.
After 48 h of hygromycin B exposure, the no observed effect concentration (NOEC) was 350 mg/l and the lowest observed effect concentration (LOEC) was 500 mg/l (Figure 2).
marmoratus embryos exposed to hygromycin B for 48 h.
marmoratus embryos and subsequent selection by hygromycin B is described here.
It is now feasible to use hygromycin B selection of potential [hyg.
Additional modifications could also be made to this protocol to reduce the amount of hygromycin B required for a similar toxic effect or transgenic selection scheme.
However, results presented here indicate the MBT is likely to be similar to other teleost fish as hygromycin B was effective at disrupting normal development in cleavage to early blastula stage embryos prior to late blastula (Figure 1E).
Pettinger RC, Wolfe RN, Hoehn MM, Marks PN, Daily WA, and McGuire JM: Hygromycin I.