The invasive glands may have more cytoplasm than adjacent AIS; however, nuclear atypia (enlargement, hyperchromasia
, variation of sizes and shapes, coarse chromatin) is usually more marked than that seen in AIS.
Tumor cells were primitive, ovoid-to-spindle or elongated in shape, dominantly with eosinophilic cytoplasm and ovoid or elongated nuclei, but without pronounced hyperchromasia
and edema, partly with hypocellular areas.
In adenoid cystic carcinomas, (1) a cribriform pattern is usually identified, (2) there is no swirling pattern, (3) nuclei are peg- or carrot-shaped and angular with nuclear hyperchromasia
, (4) glycosaminoglycan is seen in the background, and (5) there is usually an elevated mitotic index (>3/10 high-power fields).
Pathological observations: Liver of birds in groups 1-6 (Arsenic 50mg/kg) grossly revealed no significant lesions; however, microscopically liver parenchyma exhibited degenerative and necrotic changes (intracytoplasmic vacuolation) and various morphological changes in nucleus including hyperchromasia
, pyknosis, fragmentation and cells without nucleus were observed (Fig.
The villi were lined by mucin producing columnar epithelial cells with nuclear crowding, pseudostratification, and hyperchromasia
, consistent with intestinal type low grade dysplasia (Fig.
27) Cellular anaplasia, loss of polarity, discohesion, nuclear enlargement, hyperchromasia
, pleomorphism and atypical mitoses are the histopathological hallmarks of CIS.
The cytoplasm is often sparse with variable nuclear hyperchromasia
The aspirate from metastatic adenocarcinoma of liver (the most common metastatic tumour in our study) was composed of columnar cells with oval nuclie and varying degree of pleomorphism, hyperchromasia
and nucleoloar prominence with focal glandular honeycomb pattern having a dirty necrotic background.
However, standard histological evaluation for malignancy is difficult as nuclear hyperchromasia
and pleomorphism are commonly observed in both benign and malignant masses.
The cytological features reported were hyperchromasia
in the nuclei, pleomorphism and mitoses.
Histologic examination reveals bile duct hyperplasia, hyperchromasia
that involves periportal hepatocytes, hepatocytic vacuolization, and portal fibrosis.
Furthermore, they must not present atypical structures such as nuclear hyperchromasia
, atypical mitotic figures, papillary structures or psammomatous bodies; 3) The thyroid gland must be clinically normal and non-palpable (Ibrahim et al.