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immersion objective

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immersion objective [ə′mər·zhən əb‚jek·tiv]
(optics)
A high-power microscope objective designed to work with the space between the objective and the cover glass over the object filled with an oil whose index of refraction is nearly the same as that of the objective and the cover glass, in order to reduce reflection losses and increase the index of refraction of the object space. Also known as immersion lens.


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Carl Zeiss Jena produced its first oil immersion objective in 1880, designed by Ernst Abbe, who was the founder of the optical theory of microscope lenses.
CONFOCAL MICROSCOPY Series of thin optical sections (1 [micro]m apart) of villous segments lying completely inside the thick sections were recorded by a BioRad MRC 600 confocal laser scanning microscope using a planapochromat oil immersion objective with 40x magnification (N.
showed how an immersion objective permitted data acquisition from deep within papers and polymers with opaque coatings.
 
 
 
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