Of the 44 patients, 37 (84%) received a diagnosis by using [greater than or equal to] 1 of the following serologic tests for Coccidioides exposure: complement fixation, immunodiffusion
, or tube precipitin.
In the present case, studies for an infectious etiology were negative except the positive serum Coccidioides IgM/ IgG by EIA and immunodiffusion
In double immunodiffusion
test, a white band was observed between the two wells of antigen and antibodies for both the PLE-treated and piperine-treated mice serum because of the antigen-antibody reactions (Fig.
After eliminating the cross-reacting antibody using an immunoadsorbent, a strong precipitin line was observed between the egg protein and the antibody in the double immunodiffusion
test result was positive for all 5 patients.
As early as 1949, Orjan Ouchterlony used antibodies as reagents for immunodiffusion
methods (26), but the major breakthrough was with the application of RIA, which was developed by Solomon Berson and Rosalyn Yalow and for which Yalow was awarded the Nobel Prize.
The purified toxin was highly specific for antisera raised in rabbits against the purified toxin, as revealed by immunodiffusion
and immunoblot assays.
(RID) [8, 9, 10, 11] is used extensively for the quantitative estimation of antigens.
For example, it can accommodate standard pour, spiral or surface inoculated plates, as well as large bioassay and Single Radial Immunodiffusion
C3 activator, C1-INH, IgA, IgG and IgM were estimated by the single radial immunodiffusion
method of Salimonu et al.
Approximately 10 days after each injection, a blood sample was collected, the serum was separated and tested for anti-[alpha]2M antibody activity by immunodiffusion
(ID) and immunoelectrophoresis (IEP), prior and after its absorbance to normal rat serum.
The isolates were confirmed as nontoxigenic C diphtheriae by the California State Department of Public Health Microbial Diseases Laboratory (Berkeley, CA), using both PCR and the Elek immunodiffusion