immunoelectrophoresis


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Immunoelectrophoresis

A combination of the techniques of electrophoresis and immunodiffusion used to separate the components of a mixture of antigens and make them visible by reaction with specific antibodies.

A medium such as agar is deposited on a convenient base, for example, a microscopic slide. A small well is cut in the medium. A test solution is deposited in the well, and the contained substances are separated by electrophoresis along one axis of the plate. A trough is then cut in the medium parallel to, but at some distance from, the line of the separated substances. The trough is filled with antiserum which contains antibodies to one or more of the separated substances. The antiserum and substances diffuse toward one another and, where they meet, form curvilinear patterns of precipitation. These can be seen directly in clear media or can be visualized after washing out unreacted materials and staining in opaque media. See Immunoassay

immunoelectrophoresis

[¦im·yə·nō·i‚lek·trə·fə′rē·səs]
(immunology)
A serological procedure in which the components of an antigen are separated by electrophoretic migration and then made visible by immunodiffusion of specific antibodies.
References in periodicals archive ?
69) The glycoprotein abnormality can be confirmed with flow cytometry or crossed immunoelectrophoresis.
This rule applies only to the specimen and would appear not to allow ordering of an Immunoelectrophoresis on an additional blood specimen.
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Counter current line absorption immunoelectrophoresis is an alternative diagnostic screening test to counter current electrophoresis in Aleutian disease (AD) eradication programs.
Methods: Plasma samples were studied for C5a activity by granulocyte aggregation, and C3 conversion by immunoelectrophoresis.
Immunological methods for protein C measurement involve the Laurell rocket immunoelectrophoresis technique, radioimmunoassay technique or ELISA.
A diagnosis of multiple myeloma was considered, but findings on serum protein electrophoresis, urine protein electrophoresis, and urine immunoelectrophoresis were normal.
Her medical record revealed no prior evidence of hepatosplenomegaly and normal values for serum uric acid, total serum protein, serum protein electrophoresis, and immunoelectrophoresis.
Rocket immunoelectrophoresis method for the content of serum apolipoprotein A1 and B.
Serum immunoelectrophoresis and Detection of multiple myeloma and monoclonal protein evaluation; other paraproteinemic states.
The results of UPEP and urine immunoelectrophoresis studies were negative; urine total protein excretion levels were within normal limits.
San Diego, CA IMMUNOELECTROPHORESIS EQUIPMENT BioDesign Inc.

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