Immunofluorescence

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Immunofluorescence

A technique that uses a fluorochrome to indicate the occurrence of a specific antigen-antibody reaction. The fluorochrome labels either an antigen or an antibody. The labeled reactant is then used to detect the presence of the unlabeled reactant. The use of a labeled reactant (such as an antibody which both detects and indicates the antigen) to reveal the presence of an unlabeled one is termed direct immunofluorescence. The use of a labeled indicator antibody, which reacts with an unlabeled detector antibody that has previously reacted with an antigen, is termed indirect immunofluorescence. Substitution of a light meter for the human eye permits a quantitative measurement in immunofluorometry. See Immunoassay

Immunofluorescence

 

any set of methods of fluorescent analysis used in immunology, histochemistry, virology, bacteriology, mycology, and parasitology.

The combination of immunochemical reactions with fluorescence microscopy makes possible the detection of tissue and cellular antigens, including those involved in autoimmune diseases and in malignantly degenerated cells. The method is also useful in studying the patterns of antibody synthesis and in identifying the causative agents of many viral and microbial diseases. Specific antibodies are tagged with a fluorescent dye (for example, acridine orange) that will not alter their properties and then introduced to the specimen, so that only the parts of the specimen containing antigen will fluoresce. When the formation of antigen-antibody complexes is being investigated by immuno-fluorescence, the antibodies are tagged with a dye whose fluorescent properties will change when the antibodies combine with antigen.

REFERENCE

Immunofliurestsentsiia. Edited by J. Kubica. Moscow, 1967. (Translated from Polish.)

immunofluorescence

[¦im·yə·nō·flə′res·əns]
(immunology)
Fluorescence as the result of, or identifying, an immune response; a specifically stained antigen fluoresces in ultraviolet light and can thus be easily identified with a homologous antigen.
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Then immunofluorescent staining was carried out to investigate the in vitro effect of Tan IIA on the CXCR4 expression of BMSCs, which showed that Tan IIA treatment (2 [micro]M for 24 h) significantly enhanced the CXCR4 expression when compared with the control group (n = 3, Fig.
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Complement fixation, ELISA and immunofluorescent assays are commonly employed for this purpose.
ANA, anti-dsDNA, anti-Tg, and anti-TPO autoantibodies were detected with immunofluorescent assay (IFA); s[T.
Immunofluorescent antibody tests can be incorporated in cell-based assays to detect specific viruses, when required.
The most reliable and widely used test to diagnose CSD is the detection of IgM and IgG antibodies to Bartonella, using immunofluorescent assay.
To distinguish DIV from lichen planus, consider a biopsy and immunofluorescent studies to rule in or out some of the conditions in the differential diagnosis.
Demonstration of skin antibodies in sera of pemphigus vulgaris patients by indirect immunofluorescent staining.
Part II Particular techniques: Culture methods; Electrical methods; ATP bioluminescence; Microscopy techniques: DEFT and flow cytometry; Immunological techniques: immunochromatography, enzyme linked immunofluorescent assays and agglutination techniques; Immunological techniques: ELISA; Genetic techniques: PCR, NASBA, hybridisation and microarrays; Genetic techniques: molecular subtyping methods; New biosensors for microbiological analysis of food; The use of applied systematics to identify foodborne pathogens; Index.