To determine total IgE antibodies, the samples were analyzed within 24 hours by immunonephelometry
(Nephelometer Dade Behring, Marburg, Germany).
The serum concentrations of CRP were determined with immunonephelometry
, and ESR was measured using the traditional Westergren method.
hs-C-reactive protein (hs-CRP) was analyzed using the CardioPhase[R] hs-CRP (Dade Behring) 2-site particle-enhanced immunonephelometry
Serum IgG subclasses were measured by immunonephelometry
using Siemens N IgG1-2 and IgG3-4 (formerly Dade Behring, Marburg, Germany) on a Siemens BNTMII specific protein analyzer.
The detection of ESR used the Westergren method, while CRP was measured by immunonephelometry
using CRP reagents (BioSystems SA, Spain).
Cystatin C levels were assessed by immunonephelometry
on a Roche 8000 autoanalyzer (Roche, Switzerland).
CRP was assayed using particle-enhanced immunonephelometry
(Dade-Behring BN Prospec nephelometer).
Materials and Methods: The levels of serum cystatin C were evaluated in 100 subjects (50 with type 2 diabetes and 50 controls) of age group 40-70 years and were measured using particle-enhanced immunonephelometry
10,11) Serum FLC levels are determined by immunonephelometry
using sheep polyclonal antibodies that recognize epitopes on free kappa and lambda chains, which are hidden when light and heavy chains are associated in the immunoglobulin molecule.
High-sensitivity CRP (hs-CRP) was measured by immunonephelometry
Contract notice: Delivery of reagents, calibrators for the determination of specific proteins by immunonephelometry
along with renting analyzer for a period of 36 months.
Sera C3 and C4 levels analysis was done using quantitative determination by immunonephelometry
(BNProSpec, Siemens, USA).